人食管癌细胞体外光动力效应主要影响因素的实验研究  

Main factors affecting the effect of photodynamic therapy against human esophageal cancer cells in vitro

作　　者：陈晓华[1] 罗荣城[1] 李黎波[1] 丁雪梅[1] 吕成伟[1] 周小平[1] 严晓[1] 

机构地区：[1]南方医科大学南方医院肿瘤中心,广东广州510515

出　　处：《南方医科大学学报》2007年第12期1817-1820,共4页Journal of Southern Medical University

基　　金：中国临床肿瘤学科学基金(CSCO-康莱特基金)(20012378)~~

摘　　要：目的探讨光动力作用(PDT)对体外培养的人食管癌细胞Eca-109的杀伤效应,初步揭示影响Eca-109细胞光动力疗法杀伤效应的主要影响因素。方法以两种光敏剂血卟啉衍生物(HpD)和光敏素(Photofrin)不同浓度于特定光照剂量(15J/cm2)下和不同浓度的HpD在三种不同光照能量密度(10J/cm2,30J/cm2,50J/cm2)下作用于食管癌Eca-109细胞,光源采用DIOMED 630 PDT系统,24h后通过MTT法检测细胞生存率,并应用荧光分光光度计RT-5000检测不同浓度HpD作用于Eca-109细胞4h后胞内光敏剂荧光值。结果两种光敏剂不同浓度间细胞生存率均有明显差异,三种不同光照能量密度下不同HpD浓度细胞生存率间亦存在明显差异(P<0.05)。同一浓度不同光照能量密度的细胞生存率间,前4个低浓度生存率间未见明显差异(P>0.05),后4个高浓度生存率间则差异有统计学意义(P<0.05)。根据荧光分光光度计RT-5000测得的胞内荧光值及标准曲线取得胞内光敏剂浓度,并与孵育浓度进行回归分析,得相关系数r=0.997,即胞内光敏剂浓度与孵育浓度呈正相关。结论特定光源下,光照能量密度、光敏剂的种类及其光敏剂的孵育浓度是影响Eca-109 PDT效应的主要因素。Objective To evluate the tumor cell-killing effect of photodynamic therapy against human esophageal cancer cells in vitro and identify the main factors affecting the effect. Methods Human esophageal cancer Eca-109 cells were incubated for 24 h in vitro with hematoporphyrin derivative （HpD） and Photofrin at different concentrations prior to exposure to a light energy density of 15 J/cm2 delivered fi＇om a DIOMED 630 PDT system. The cell killing effect was also evaluated for different HpD concentrations combined with 3 light energy densities （10, 30, and 50 J/cm2）, respectively. The cell survival rate was measured using MTT assay, and fluorescence spectrometry was used to detect the intracelhilar photosensitizer fluorescence of the tumor cells after incubation with HpD for 4 h. Results The cell survival rate after incubation with the two ph0tosensitizers at different concentrations were significantly different, and under the 3 different light energy densities, incubation of the cells with different HpD concentrations also resulted in significantly different cell survival rates （P〈0.05）. At the 4 low photosensitizer concentrations and with different light energy densities, the cellsurvival rates were similar （P〉0.05）, but the 4 higher photosensitizer concentrations resulted in significant difference in the cells survival （P〈0.05）. Correlation analysis showed that the intracellular photosensitizer concentration was positively correlated to the photosensitizer concentrations in cell incubation （r=0.997）. Conclusion When the light source remains constant, the light energy density, the kinds of photosensitizers and their concentrations are the main factors affecting the Eca-109 cell-killing effect of PDT.

关 键 词：血卟啉衍生物 光动力疗法 ECA-109 细胞内光敏剂浓度 细胞生存率 

分 类 号：R735.1[医药卫生—肿瘤]