免疫亲和-光化学柱后衍生高效液相色谱荧光法测定花生粕中黄曲霉毒素的研究  

作　　者：陈新[1] 王雄 雷达[2] 

机构地区：[1]中国农业科学院农业质量标准与检测技术研究所 [2]北京中检维康技术有限公司

出　　处：《中国饲料》2007年第24期30-31,34,共3页China Feed

基　　金：国家科技支撑计划项目;项目编号:2006BAD12B03

摘　　要：研究建立了光化学柱后衍生-高效液相色谱(HPLC)-荧光检测器测定花生粕中黄曲霉毒素B1、B2、G1和G2的方法。甲醇/水提取样品中黄曲霉毒素B1、B2、G1和G2,Afla-P黄曲霉毒素免疫亲和柱净化,经高效液相色谱分离后,由光化学柱后衍生,通过荧光检测器测定。结果表明:在优化条件下,黄曲霉毒素B1、B2、G1和G2的检出限分别为1.0、0.3、1.0μg/kg和0.3μg/kg,回收率为67.0%～117%,相对标准偏差(RSD)低于18.2%。该方法简便快速、灵敏度高、重现性好,满足饲料用花生粕中黄曲霉毒素检测的需要。A method for determination of Aflatoxin B1 ,B2,G1 and G2 by using photochemical derivatization-HPLC- fluorescence detector was developed.The Aflatoxin B1,B2,G1 and G2 in samples were extracted by methanol / water and were cleaned up by immuno affinity columns.The separation of Aflatoxin B1,B2,G1 and G2 was conducted by HPLC,the determination was carried out by fluorescence detector after photochemical defivatization.Under optimum conditions,the limits of determination of Aflatoxin Bl ,B2,G1 and G2 were 1.0,0.3,1.0 μg/kg and 0.3 μg/kg respectivdy,the recoveries of analytes were from 67.0 % to 117 % and the relative standard deviations were below 18.2 %.The method is simple,high sensitive and good repeatability, which is suitable for the determination of Aflatoxin in peanut residues.

关 键 词：免疫亲和柱 光化学衍生 高效液相色谱 黄曲霉毒素 花生粕 

分 类 号：S816.17[农业科学—饲料科学]