鹿茸多肽对四氯化碳所致小鼠急性肝损伤的保护作用  

Protective Effect of Velvet Antler Peptides on Acute Hepatic Injury by Carbon Tetrachloride

作　　者：李夏[1] 段冷昕[1] 王楠娅[2] 金瑾[1] 宋宇[3] 周秋丽[1] 

机构地区：[1]吉林大学药学院,长春130021 [2]吉林大学第一医院血液肿瘤科,长春130021 [3]吉林大学畜牧兽医学院,长春130012

出　　处：《中国药学杂志》2007年第24期1864-1866,共3页Chinese Pharmaceutical Journal

基　　金：国家863资助项目(2006AA03Z328)

摘　　要：目的观察梅花鹿茸多肽(VAP)对四氯化碳(CCl4)所致小鼠急性肝损伤的保护作用。方法腹腔注射四氯化碳制备小鼠急性肝损伤模型,检测血清谷丙转氨酶(ALT)、谷草转氨酶(AST)活性和白蛋白(ALB)含量;同时测定肝组织中丙二醛(MDA)含量和超氧化物歧化酶(SOD)、谷光甘肽(GSH)的活性。在光学显微镜下观察肝脏病理组织学变化。结果与四氯化碳模型组比较,VAP可使血清ALT,AST活性明显降低(P<0.01),但对ALB的含量无明显影响(P>0.05);同时VAP可使肝组织中MDA的含量明显下降(P<0.01),GSH含量有所升高,SOD活力明显增强(P<0.01)。形态学观察,VAP能明显改善肝组织的病理学改变。结论VAP对四氯化碳所致小鼠急性肝损伤具有保护作用。OBJECTIVE To study the protective effects of velvet antler polypeptides（VAP） on acute hepatic injury induced by carbon tetrachloride（CCl4） in mice.METHODS Sixty Kunming mice were randomly divided into 6 groups.The VAP groups were treated with VAP at a dose of 12.5,25 and 50 mg·kg^-1·d^-1 for 5 days subcutaneously.At the same time,the normal control group and the model control group were given equivalent amount of 0.9% sodium chloride solution by the same route.And mice in the positive control group were intragastically administrated with 200 mg·kg^-1·d^-1 BDD.After the last administration,all groups except the normal control were intraperitoneally injected with 0.2% CCl4 solution to induce acute hepatic injury.Thereafter, the contents of ALT,AST,ALB in the serum,MDA and the activity of SOD,GSH in the liver were measured and the hepatic histological changes were observed by optical microscope.RESULTS Compared with the model group, the contents of ALT,AST in the serum was obviously decreased（P〈0.01,P〈0.001）,the content of MDA in the liver was also content lowered（P〈0.01）,and the activity of GSH and SOD in the liver was markedly improved（P〈0.05,P〈0.01） after preventive treatment with VAP.VAP Ameliorated the hepatic pathological changes.CONCLUSION VAP have protective effects on acute hepatic injury induced by CCl4 in mice.

关 键 词：鹿茸 多肽 急性肝损伤 四氯化碳 

分 类 号：R969.4[医药卫生—药理学]