高效液相色谱同时测定食品中BHA、BHT、TBHQ方法的研究  

Determination of BHA,BHT and TBHQ in foods by high-performance liquid chromatography

作　　者：余涛[1] 叶坚[1] 

机构地区：[1]广东省肇庆市疾病预防控制中心,广东肇庆526020

出　　处：《中国卫生检验杂志》2007年第12期2136-2137,2155,共3页Chinese Journal of Health Laboratory Technology

摘　　要：目的：探讨液相色谱测定食品中BHA、BHT、TBHQ的方法。方法：采用甲醇分次萃取样品，提取液定容后用中性氧化铝吸附杂质，最后于液相色谱仪进行分析定量。结果：BHA、BHT、TBHQ在0～80μg／ml浓度范围内，标准曲线线性关系良好，相关系数r分别为0．9996、0．9998、0．9999；BHA的相对标准偏差为1．2％～4．9％，BHT的相对标准偏差为1．7％～6．1％，TBHQ的相对标准偏差为2．1％～6．2％；BHA的加标回收率为94．4％～102．4％，BHT的加标回收率为94．0％～98．5％，TBHQ的加标回收率为92．4％～101．0％；BHA、BHT检测结果与国标方法比较无显著性差异（P〉0．05）。结论：本方法不但简便、快捷，适合开展大批量检测工作，而且精密度、准确性均满足检测要求，此外对保障实验操作人员的身体健康也有积极的作用。Objective ：To develope a method for the determination of BHA, BHT and TBHQ in foods by using high-perform-ance liquid chromatography. Methods：The BHA, BHT and TBHQ were extracted from samples with the methanol, and analyzed by high-performance liquid chromatography. Results： The linear ranges of determination for BHA, BHT and TBHQ were 0 -80μg/ml（r=O.9996）, 0-80 μg/ml （r=O.9998） ,0-80μg/ml （r=O.9999） , respectively. The relative standard derivations for BHA, BHT and TBHQ were 1.2%-4. 9%, 1.7%-6. 1% , 2. 1% - 6. 2% , respectively. The recoveries of samples for BHA, BHT and TBHQ were 94. 4% - 102.4% ,94. 0% - 98.5% ,92.4% - 101.0%, respectively. Conclusion： The method has the advantages of simplicity, repeatability and sessitivity and can be used for simultaneous analysis of BHA, BHT and TBHQ in foots and protect the health of worker.

关 键 词：叔丁基羟基茴香醚 2 6-二叔丁基羟基甲苯 特丁基对苯二酚 高效液相色谱 

分 类 号：O657.72[理学—分析化学]