应用脉冲场凝胶电泳分型技术溯源副溶血弧菌食物中毒  

Application of pulse-field gel electrophoresis analysis in source-tracking of food-borne disease caused by Vibrio parahaemolyticus

作　　者：邓志爱[1] 李孝权[1] 胡玉山[1] 刘俊华[1] 张欣强[1] 林云万[1] 莫自耀[1] 

机构地区：[1]广州市疾病预防控制中心微生物检验科,510080

出　　处：《中华预防医学杂志》2008年第1期36-38,共3页Chinese Journal of Preventive Medicine

基　　金：广州市科技计划资助项目（2005J1-co191）;广州市卫生局医药卫生科技资助项目（2006-Zdi-11）

摘　　要：目的应用脉冲场凝胶电泳（PFGE）技术对副溶血弧菌食物中毒进行分析。方法采用限制性内切酶NotⅠ，对一起广州市某餐厅副溶血弧菌食物中毒中30株副溶血弧菌进行PFGE分子分型，用BioNumerics Version4．0软件（复选Dice相关系数和UPGMA方法）进行聚类分析。结果分离的30株副溶血弧菌特异性toxR基因均为阳性，毒力基因tdh阳性，而trh阴性。30株副溶血弧菌PFGE型别相同。结论PFGE分型可揭示人、食品和公用具分离的副溶血弧菌菌株之间的流行病学联系，为疫情溯源提供分子流行病学证据和支持。Objective To apply pulse-field gel electrophoresis analysis（PFGE） in analysing a case of food poisoning caused by Vibrio parahaemolyticus. Methods PFGE using restriction enzyme Not I was employed in molecular subtyping of thirty strains of V. parahaemolyticus isolated from a case of food poisoning in Guangzhou city and PFGE patterns were analyzed by using BioNumerics Version 4.0 software to perform duster analysis. Pattern profiles were compared by using the Dice coefficient and unweighted pair group method with arithmetic averages （UPGMA）. Results Thirty strains were of the same type of pulsotype. Conclusions Molecular subtyping by PFGE might disclose the epidemiological relationships of the strains from humans ,food and the environment, giving a strong molecular epidemiological evidence and a support for the source-tracking of outbreak events.

关 键 词：副溶血弧菌 电泳 凝胶 脉冲场 细菌分型技术 食物中毒 

分 类 号：R686[医药卫生—骨科学]