胶质瘤中膜型基质金属蛋白酶1mRNA水平表达  

Study of mRNA expression level of gene MT1-MMP in glioma

作　　者：刘维生[1] 肖新如[1] 耿会娟[2] 陈善文[1] 王硕[1] 赵继宗[1] 

机构地区：[1]首都医科大学附属北京天坛医院神经外科 [2]首都医科大学附属北京天坛医院检验科,100050

出　　处：《北京医学》2008年第1期1-4,共4页Beijing Medical Journal

摘　　要：目的探讨胶质瘤恶性程度和膜型基质金属蛋白酶1(MT1-MMP)mRNA表达水平的关系,为胶质瘤的诊断、治疗及预后等提供参考。方法分别采用实时荧光定量逆转录PCR法(RT-PCR)和半定量RT-PCR法检测34例胶质瘤和7例正常脑组织中MT1-MMPmRNA的表达水平。结果实时荧光定量RT-PCR法检测正常脑组织和II级(低级别)、III级、IV级、高级别(III、IV级)胶质瘤的△△Ct值分别为0.834±0.517,4.063±2.309,6.376±4.023,7.653±4.196和7.072±4.072。半定量RT-PCR法测定结果显示,正常脑组织和II级、III级、IV级、高级别胶质瘤的相对灰度值分别为0.799±0.156,1.142±0.158,1.538±0.482,1.652±0.376和1.600±0.421。正常脑组织和II、III、IV级胶质瘤之间,II、IV级胶质瘤之间,正常脑组织和低、高级别胶质瘤之间,低、高级别胶质瘤之间MT1-MMP基因mRNA表达水平均有显著性差异。结论胶质瘤中存在MT1-MMP基因mRNA高水平的表达,随着肿瘤恶性程度的增加,MT1-MMP基因mRNA的表达水平增高。Objective To analyze the relationship between malignancy of glioma and mRNA expression level of gene MT1-MMP and provide information for clinical diagnosis, therapy and prognosis of the evaluation. Methods The mRNA expression level of gene MT1-MMP in 34 gliomas and 7 normal brain tissues were detected with quantitative realtime RT-PCR and semi-quantitative RT-PCR. Results With Real-time RT-PCR method, the A ACt value of normal brain tissue and grade Ⅱ （low-grade）,Ⅲ、Ⅳ high-grade glioma were 0.834±0.517, 4.063±2.309, 6.376＋4.023,7.653＋ 4.196,7.072＋4.072 respectively. With semi-quantitative RT-PCR method, the relative value of normal brain tissue and grade Ⅱ （low-grade）,Ⅲ,Ⅳ,high-grade glioma were 0.799＋0.156, 1.142＋0.158, 1.538＋0.482,1.652＋0.376, 1.600＋0.421 respectively. There was the statistical significant difference between normal brain tissue and grade Ⅱ ,Ⅲ ,Ⅳglioma, grade Ⅱ and grade Ⅳglioma, normal brain tissue and low-grade,high-grade glioma, low-grade and high-grade glioma. Conclusions The mRNA expression of gene MT1-MMP is up-regulated in glioma, and the mRNA expression of gene MT1-MMP increases with the malignancy of glioma.

关 键 词：胶质瘤 膜型基质金属蛋白酶1 实时荧光定量逆转录-聚合酶链式反应 半定量逆转录-聚合酶链式反应 

分 类 号：R739.4[医药卫生—肿瘤]