TFPI-2对胰腺癌细胞体内和体外侵袭能力的影响  

Effect of Recombinant Tissue Factor Pathway Inhibitor 2 Gene on The Invasion of Human Pancreatic Cancer in Vitro and in Vivo

作　　者：汤志刚[1] 孙振阳[1] 胡何节[1] 许戈良[1] 陈炯[1] 李建生[1] 

机构地区：[1]安徽医科大学附属安徽省立医院普通外科,合肥230001

出　　处：《中国普外基础与临床杂志》2008年第1期46-50,共5页Chinese Journal of Bases and Clinics In General Surgery

基　　金：安徽省自然科学基金资助项目(编号:050430702)~~

摘　　要：目的研究组织因子途径抑制物-2(TFPI-2)基因对人胰腺癌细胞系Panc-1细胞侵袭能力的影响。方法通过脂质体法将TFPI-2基因转染到Panc-1细胞,用RT-PCR和Western blot分别检测转染前、后TFPI-2mRNA和相应蛋白的表达。Boyden小室法测定Panc-1-TFPI-2、Panc-1-V和Panc-1-P3组细胞的体外侵袭能力的变化,同时将3组细胞分别接种裸鼠,观察其体内肿瘤生长及转移情况。结果转染成功的Panc-1细胞有TFPI-2mRNA及相应蛋白的表达;Panc-1-TFPI-2组、Panc-1-V组和Panc-1-P组细胞穿膜细胞数分别为(24.4±3.5)个、(61.3±4.1)个和(60.2±3.9)个,前者明显少于后两者(P<0.05),提示Panc-1-TFPI-2组细胞的体外侵袭能力下降。3组细胞分别接种裸鼠,Panc-1-TFPI-2组肿瘤体积为(438.0±69.8)mm3,与Panc-1-V组的(852.0±102.9)mm3和Panc-1-P组的(831.0±78.1)mm3相比明显缩小,差异有统计学意义(P<0.05);镜下见Panc-1-V组和Panc-1-P组肿瘤组织浸润到肌层,有肝、肺转移灶,而Panc-1-TFPI-2组未见肌层浸润及肝、肺转移。结论TFPI-2基因表达可抑制胰腺癌细胞系Panc-1的侵袭转移能力,为胰腺癌的基因治疗提供了实验依据。Objective To investigate the invasion ability of Panc-1 cells in vivo and in vitro after being transfected with tissue factor pathway inhibitor 2 gene （TFPI-2）. Methods The expression vector pEGFP-C1-TFPI-2 was transfected into human pancreatic cancer line Panc-1 cells by using liposome. TFPI-2 mRNA and protein of transfected and nontransfected cells were detected by reverse transcription-polymerase chain reaction （RT-PCR） and Western blot respectively. The tumor cells invasive behavior of transfected （Panc-I-TFPI-2） and nontransfected （Panc-1-V and Panc-1-P） cells were assessed in vitro through Boyden Chamber method. The transfected and nontransfected cells were implanted into nude mice to observe its growth and metastasis in vivo. Results Expressions of mRNA and protein of TFPI-2 were confirmed in transfected cells. After TFPI-2 transfection, the number of Panc-I-TFPI-2, Panc-1-V and Panc-1-P cells passing through membrane of Boyden Chamber were 24. 4± 3. 5, 61.3±4.1 and 60.2±3.9, respectively. The number of TFPI-2-expressing cells to traverse a Matrigel-coated membrane was obviously decreased compared with that of non-expressing cells, the invasion ability was lower than that before transfection in vitro. The subcutaneous tumor volume of the Panc-I-TFPI-2 group was （438.0±69.8） mm3 , the Panc-1-V group was （852.0±102.9） mm3 and the Panc-1-P group was （831.0±78. 1） mm3 , P〈0.05. The metastasis to liver and lung and muscular invasion occurred in the Panc-1-V group and the Panc-1-P group. There were no muscular invasion and metastatic lesions in the Panc-1-TFPI-2 group. Conclusion TFPI-2 gene expression may obviously inhibit the invasion ability of pancreatic cancer cells in vitro and in vivo, which provides an experi- mental basis for the treatment of human pancreatic cancer by gene therapy.

关 键 词：胰腺癌 肿瘤转移 肿瘤浸润 组织因子途径抑制物-2 

分 类 号：R735.9[医药卫生—肿瘤]