两株细菌与丛枝菌根真菌联合接种对红壤中DEHP降解的影响  

EFFECT OF COMBINED INCUBATION OF TWO BACTERIA STRAINS AND AN ARBUSCULAR MYCORRHIZAL FUNGI ON DEHP DEGRADATION AND GROWTH OF MUNG BEAN IN RED SOIL

作　　者：秦华[1] 林先贵[1] 尹睿[1] 张华勇[1] 王俊华[1] 

机构地区：[1]中国科学院南京土壤研究所,南京210008

出　　处：《土壤学报》2008年第1期143-149,共7页Acta Pedologica Sinica

基　　金：国家自然科学青年基金项目(40101015)资助

摘　　要：采用绿豆为供试植物,通过温室盆栽试验研究接种邻苯二甲酸二(2-乙基己基)酯(DEHP)降解菌与丛枝菌根(Arbuscular mycorrhiza,AM)真菌对DEHP污染土壤的修复作用以及对植物生长的影响。试验土壤中添加DEHP浓度为100mg kg-1,试验设AM真菌Acaulospora 90034、降解菌Bacillus sp.DW1和Gordonasp.DH3单独接种以及互相组合的联合接种处理,同时设置不接种的对照处理(CK)。苗后60d收获植株。结果表明,AM真菌能很好地侵染绿豆的根系。菌根侵染提高了绿豆植株的干重,同时也促进了绿豆的磷营养,但接种DW1与DH3对菌根侵染率与绿豆生长都没有显著影响。三种菌剂无论是单独或者联合接种都能显著促进土壤中DEHP的降解,但三种菌剂同时接种对DEHP的降解能起到最好的协同作用。同时,接种AM真菌也减少了DEHP在绿豆地上部分的累积,这些都为DEHP污染农田土壤的生物修复提供了理论依据。A pot culture experiment was carried out to study effect of combined incubation of DEHP degrading bacteria and arbuscular mycorrhizal （AM） fungi on bioremediation of DEHP polluted soil and growth of mung bean. Two bacterial strains, i. e. Bacillus sp. DW1 and Gordona sp. DH3, and one strain of AM fungi, Acaulospora laevis 90034, were inoculated, alone or in combination, to the soil amended with 100 mg kg^-1 DEHP. A treatment of uninoculated soil was set as control （CK）. The plants were harvested 60 days after seedling emergence. Results of the experiment show that AM fungi colonized well in the roots of mung bean. Dry mass of the plant was increased and P nutrition improved by AMF inocula. But inoculation of just DW1 and DH3 did not show any significant effect on mycorrhizal colonization and plant growth. However, the three strains of inoculants, whether inoculated alone or in combination, could obviously promote degradation of DEHP in the soil, but the best synergistic effect on DEHP degradation was found when strains DW1, DH3 and AM fungi wer inoculated in combination. Besides, the inoculation of AMF decreased DEHP accumulation in the shoots of mung bean. The findings in the experiment may serve as scientific basis for bioremediation of DEHP-contaminated soils.

关 键 词：绿豆 丛枝菌根真菌 细菌 邻苯二甲酸二异辛酯 联合接种 

分 类 号：X172[环境科学与工程—环境科学]