检索规则说明:AND代表“并且”;OR代表“或者”;NOT代表“不包含”;(注意必须大写,运算符两边需空一格)
检 索 范 例 :范例一: (K=图书馆学 OR K=情报学) AND A=范并思 范例二:J=计算机应用与软件 AND (U=C++ OR U=Basic) NOT M=Visual
机构地区:[1]山东大学生命科学院微生物技术国家重点实验室,济南250100
出 处:《微生物学通报》1997年第2期99-103,共5页Microbiology China
基 金:国家及山东省自然科学基金
摘 要:以pUC9质粒DNA作为载体,用PstⅠ酶切、碱性磷酸酯酶处理后,与PstⅠ部分酶切的多能硫杆菌染色体DNA3-10kbDNA片段连接,转化大肠杆菌JM83,在MacConkey培养基上筛选重组于,所得的重组子为6.5×103,达到建库要求的理论值。进一步用光合细菌Rhodobactersphaeroides类型Ⅱ磷酸核酮糖羧化酶/加氧酶基因(rbcL-rbcS)为探针,从该库中筛选到了含有多能硫杆菌的1,5-二磷酸核酮糖羧化酶/加氧酶(RubisCO)基因片段的重组子,从而进一步证明所构建多能硫杆菌基因文库的完整性和可靠性。The genomic library of Thboacdha mptus was constructed by using pUC9 plasmid as vecter. pUC9 was digested by PstI enzyme, treated with phosphatase, then ligated with 3~10kb fragments of T. versutus partially digested by PstI and transformed to hota coli JM83. The recombinant were screened on MacConkey medium and 6.5 ×103 recombinants were obtained. It has exceeded the necessary number of recombinants that represented an entire genom of T.versutus.Furthermore the recombinant containing the gene fragment of ribulose 1, 5-bisphosphate carbooxylase/oxygenase was isolated from the library.
正在载入数据...
正在载入数据...
正在载入数据...
正在载入数据...
正在载入数据...
正在载入数据...
正在载入数据...
正在链接到云南高校图书馆文献保障联盟下载...
云南高校图书馆联盟文献共享服务平台 版权所有©
您的IP:216.73.216.15