三硝基苯磺酸/乙醇与免疫复合物联合诱导的结肠炎动物模型  

Establishment of rat colitis model induced by trinitro-benzene-sulfonic acid and immunocomplex

作　　者：谭琰[1] 邹开芳[1] 钱伟[1] 

机构地区：[1]华中科技大学同济医学院协和医院消化内科

出　　处：《胃肠病学和肝病学杂志》2008年第1期46-49,共4页Chinese Journal of Gastroenterology and Hepatology

摘　　要：目的建立三硝基苯磺酸/乙醇与免疫复合物联合诱导的结肠炎动物模型,探讨炎症性肠病的发病机制。方法SD大鼠168只,雌雄各半,分为:①正常对照组42只,②三硝基苯磺酸/乙醇模型组42只,③免疫复合物组42只,④三硝基苯磺酸/乙醇与免疫复合物联合诱导的结肠炎动物模型组42只(以上每组雌雄各半)。分别于造模后1、2、3、4、6、9、12周末将大鼠处死(每组每周处死6只)。观察各组结肠大体形态和组织学改变,并检测肠黏膜髓过氧化物酶(MPO)活性,大便潜血实验。结果三硝基苯磺酸/乙醇与免疫复合物联合诱导的结肠炎动物模型组大鼠大体形态损伤评分均数为5·8571±0·8431;组织学损伤评分均数为4·8571±0·3542;MPO均数为3·8588±0·8625;OBT积分均数为4·1429±0·6010皆明显高于其余各组,具有统计学意义。结论上述联合诱导的结肠炎动物模型是一种较理想的IBD动物模型,可作为研究IBD发病机制及评估药物疗效的有益工具。Objective To make colitis model induced by trinitro-benzene-sulfonie acid（TNBS） and immunocomplex in rat and to assess its value in research study of inflammatory bowel disease. Methods One hundred and sixty-eight Sprague-Dawley rats, female and male hold a half each other, which were divided into four groups.① Forty two rats as control group, isotonic Na chloride was instilled into rats colon according to 100 mg/kg. ② Forty two rats as TNBS/ethanol group, which was instilled TNBS dissolved in 50% ethanol according to 100 mg/kg. ③ Forty two rats as immunocomplex group, which antigen-emolsifier contained antigen of 8 mg was injected into digiti pedis and fold inguen, interval time was two weeks. ④Forty two rats as TNBS/alcohol and immunocomplex group, which was injected antigenemolsifier firstly. We observed macroscopical, histological change, myeloperoxidase （MPO） activity and fecal occult blood test （OBT） at the 1, 2, 3, 4, 6,9,12 weekend after model developed. Results The mean of macroscopical, histological change, MPO activity and OBT of TNBS/ethanol and immunocomplex rats were 5. 8571 ± 0. 8431, 4.8571 ±0. 3542,3. 8588±0. 8625,4. 1429 ±0. 6010 respectively, which was signifieently increased than other groups. Conclusion TNBS/ethanol and immunocomplex induced rat distal colitis is an ideal model of IBD and may serve as a tool for investigation of the pathogenesis and pharmaceutical effect of IBD.

关 键 词：三硝基苯磺酸(TNBS) 免疫复和物(抗原乳化剂) Sprague—Dawley大鼠 结肠炎 

分 类 号：R574.62[医药卫生—消化系统]