Aurora A在人前列腺癌中的表达  

Effect of Aurora A on Carcinogenesis of Human Prostate Cancer

作　　者：屈艺[1] 张林[1] 母得志[1] 黄翔[2] 魏大鹏[3] 赵凤艳[3] 刘柏林[3] 

机构地区：[1]四川大学华西第二医院实验中心,成都610041 [2]四川省人民医院泌尿外科 [3]四川大学华西基础医学与法医学院生物化学与分子生物学研究室

出　　处：《四川大学学报（医学版）》2008年第1期1-5,共5页Journal of Sichuan University(Medical Sciences)

基　　金：国家自然科学基金(批准号2002AA24101,30570623);教育部博士点基金(20050610094)

摘　　要：目的观察Aurora A在人前列腺癌中的表达情况,探讨Aurora A是否在前列腺癌发生发展中起一定作用。方法采用RT-PCR、免疫组织化学和Western blot,检测在前列腺癌组织及其细胞系中Aurora A mRNA和蛋白表达。同时,在前列腺癌细胞系PC3中导入靶向Aurora A mRNA的DNA核酶,观察抑制Aurora A对前列腺癌细胞生长的影响。结果91%的前列腺癌组织和三种前列腺癌细胞系(PC3、LNCaP和Du145)中Aurora A表达阳性,而且,靶向Aurora A mRNA的DNA核酶导入PC3细胞后抑制了Aurora A表达,使PC3细胞停滞在G2/M期,并促进细胞凋亡。结论Aurora A可能在前列腺癌形成中起一定作用,Aurora A可望成为前列腺癌治疗中有价值的靶点。Objective To investigate whether Aurora A is involved in prostate cancer carcinogenesis Methods The expressions of Aurora A mRNA and protein in prostate cancer tissue and cell line were measured using RT-PCR, immunohistochemistry and Western blot analyses. Meanwhile, A DNAzyme targeting at Aurora A mRNA was performed to detect the inhibition effect of Aurora A in regulating the growth of prostate cancer cell line PC3. Results Aurora A expression was up-regulated in 91% prostate cancer tissue as well as in prostate cancer cell lines such as PC3, LNCaP and Du145. Furthermore, Aurora A expression suppressed by cell transduction of a DNAzyme targeting at Aurora A mRNA made the PC3 cells both arrest the cell cycle in G2/M phase and increase the cell apoptosis. Conclusions These findings suggest that Aurora A up-regulation may be involved in carcinogenesis of prostate cancer, and that aurora A gene may be a valuable target for treatment of prostate cancer.

关 键 词：AURORA A基因表达 前列腺癌 DNA核酶 

分 类 号：R737.25[医药卫生—肿瘤]