JAK激酶抑制剂AG490对乳腺癌细胞侵袭转移的影响  

Effect of Janus Kinase Inhibitor AG490 on Invasion and Metastasis of Human Breast Cancer Cells

作　　者：陈红芳[1] 邓华瑜[1] 范维珂[1] 

机构地区：[1]重庆医科大学病理生理学教研室,重庆400016

出　　处：《四川大学学报（医学版）》2008年第1期63-65,83,共4页Journal of Sichuan University(Medical Sciences)

基　　金：重庆市科技计划项目(CSTC;2005AC0075)

摘　　要：目的研究JAK激酶抑制剂AG490对乳腺癌细胞MDA-MB-231侵袭转移的影响,探讨JAK-STAT3信号转导通路在乳腺癌侵袭转移调控中的作用。方法以乳腺癌细胞MDA-MB-231为研究对象,应用JAK激酶抑制剂AG490处理细胞,用MTT法检测细胞对人工基底膜的粘附能力变化;Transwell小室进行人工重组基底膜侵袭和运动实验;Western blot检测细胞中P-STAT3水平。结果JAK激酶抑制剂AG490可使人乳腺癌细胞MDA-MB-231中P-STAT3表达减弱,同时可使细胞粘附、侵袭、迁移能力下降。结论JAK-STAT3信号转导通路参与乳腺癌细胞侵袭转移调控,阻断通路活化可以抑制乳腺癌细胞的侵袭转移。Objective To investigate the effect of Janus kinase （JAK） inhibitor AG490 on invasion and metastasis of the human breast cancer cell MDA-MB-231, and to explore the regulating role of JAK-STAT3 signaling pathway when the breast cancer occurs to the invasiveness and metastasis. Methods The human breast cancer cell MDA-MB-231 was used as the research object, and AG490 was as Janus kinase inhibitor. The adhesion of MDA-MB-231 cell attaching to matrigel was measured with MTT assay. The invasion and metastasis potential were evaluated with transwell chamber. The P-STAT3 protein in cell was detected by Western-blot. Results Janus kinase inhibitor AG490 could make that the expression of P-STAT3 in human breast cancer cell MDA-MB- 231 became weak, and that the abilities of adhesion, invasion and metastasis were also dropping down as compared with the control group （P〈0. 01）. Conclusion JAK-STAT3 signaling pathway participates in regulating the invasion and metastasis of human breast cancer. Inhibiting the activation of JAK-STAT3 signaling pathway can suppress the invasion and metastasis of human breast cancer.

关 键 词：乳腺癌 JAK-STAT3 AG490 侵袭 转移 

分 类 号：R737.9[医药卫生—肿瘤]