褪黑素对离体帕金森病模型黑质NF-κB表达及黑质细胞凋亡的影响  

Protective Effect of Melatonin on PD the Substantia Nigra Slices Induced by 6-OHDA(in vitro)

作　　者：邢红霞[1] 彭海[2] 刘敏[3] 王玉梅[1] 朱红灿[4] 

机构地区：[1]新乡医学院第一附属医院神经内一科,河南卫辉453100 [2]华中科技大学同济医学院协和医院神经内科,湖北武汉430022 [3]武汉市精神卫生中心神经内科,湖北武汉430022 [4]郑州大学第一附属医院神经内科,河南郑州450052

出　　处：《中国神经免疫学和神经病学杂志》2008年第1期9-11,14,共4页Chinese Journal of Neuroimmunology and Neurology

基　　金：国家自然科学基金资助项目(30300114)

摘　　要：目的研究褪黑素(melatonin,MT)对6-羟多巴胺(6-hydroxydopamine,6-OHDA)所制造的离体帕金森病(Pakinson disease,PD)模型的影响。方法将14只大鼠分成假手术组、MT组和对照组3组,MT组大鼠经腹腔连续3 d注射MT,另两组以相同方法注射等量生理盐水,然后MT组和对照组分别取脑片置于含6-OHDA的人工脑脊液中孵育2 h。应用TUNEL法、免疫组化技术观察黑质细胞凋亡的数量并检测黑质细胞NF-κBp65表达情况。结果假手术组未见明显黑质细胞凋亡,MT组较对照组黑质细胞凋亡明显减少,且黑质细胞NF-κB p65的表达也有所减少,与对照组比较差异具有统计学意义(P<0.05)。结论MT对离体PD模型具有保护作用,其机制可能为抗凋亡。Objective To study the protective effects and mechanism of melatonin （MT） precondition on Parkinson disease （PD） brain slice of substantia nigra （SN, in vitro） induced by 6 hydroxydopamine （6-OHDA）. Methods 14 rats were divided into 3 groups： The sham-operation group, MT group and control group. Melatonin was administered intraperitoneally to MT group and 0. 9% sodium chloride to control group or sham operation group for three consecutive days. The PD brain slices of SN were incubated by 6-OHDA for 2 hours. Immolunohistochemical technique was used to observe the apoptosis neuronal number of SN and the changes of the expression of NF-kB p65. Results The neuronal number of SN in apoptosis induced by 6-OHDA showed significantly decreasing in the MT precondition group （P〈0.05）. The expression of NF-kB p65 was also decreased significantly in the MT group. Conclusions MT may prevent neurodegeneration and play a protective role in dopaminergic neurons, and the neuro-protective mechanism of the may be involved with anti apoptosis.

关 键 词：帕金森病 6-羟多巴胺 黑质细胞 凋亡 核因子-KB p65 

分 类 号：R742.5[医药卫生—神经病学与精神病学]