中药复健片对大脑中动脉闭塞大鼠脑多唾液酸-神经细胞黏附分子表达的影响  

Effect of Chinese Herb Fujian Tablets on the Expression of Polysialic Acid-neural Cell Adhesion Molecule in the Brain of Rats with the Middle Cerebral Artery Occlusion Rat

作　　者：周永红[1] 王新陆[2] 张玉杰[1] 胡怀强[2] 

机构地区：[1]青岛大学医学院中医教研室,山东青岛266021 [2]山东中医药大学临床学院,山东济南250014

出　　处：《中国神经免疫学和神经病学杂志》2008年第1期33-35,38,共4页Chinese Journal of Neuroimmunology and Neurology

基　　金：山东省科技发展计划基金资助项目(2006GG3202005)

摘　　要：目的观察中药复方复健片对大脑中动脉闭塞模型大鼠脑内多唾液酸-神经细胞黏附分子(PSA-NCAM)的影响,探讨其促进神经发生的作用机制。方法60只Wistar大鼠随机分为药物组、模型组、假手术组,每组20只。用Tamura等方法制备大脑中动脉闭塞大鼠模型,于造模成功后6 h药物组按体重9 g/kg经灌胃给予复健片水溶液,余两组分别经灌胃给予同等量生理盐水(NS),1次/d,共7 d。用免疫组化法观察大脑中动脉闭塞模型大鼠脑内PSA-NCAM的表达,并对切片进行图像分析,测定染色平均灰度。结果56只大鼠纳入结果分析。给药1周后大鼠脑内PSA-NCAM染色平均灰度药物组为100.10±2.28,模型组为128.65±1.12,假手术组为140.20±8.68。药物组、模型组与假手术组组间比较差异有统计学意义(P<0.01),药物组与模型组比较差异有统计学意义(P<0.01)。结论复健片可显著增强大脑中动脉闭塞大鼠脑内PSA-NCAM的表达,从而促进缺血性脑卒中神经的发生。Objective To study the effect of complex of the Chinese herb Fujian （CCF）tablets on the expression of polysialic acidneural cell adhesion molecule （PSA-NCAM） in the brain of rats with middle cerebral artery occlusion （MCAO） and explore its action mechanism to promote neurogenesis. Methods Totally 60 rats were randomly divided into the CCF drug group, MCAO model group and sham-operation group with 20 rats in each one . The rat models of MCAO were successfully established by the Tamura method. 6 hours later, the rats of CCF group were given 9 g/kg of a CCF tablets in liquicl form for oral use, and the rest groups were given oral normal saline at the same volume once a day for 1 week. The expression of PSA-NCAM in the brain of MCAO rats was observed by immunohistochemical method and the brain sections were subjected to image analysis for determine mean staining gray. Results Fifty six rats were involved in the result analysis. After 1 week of administration, the mean gray value of the expression of PSA-NCAM in the CCF group was 100.10±2.28, the MCAO group was 128.65±1.12, the sham-operation group was 140.20±8.68 respectively. The CCF group and the MCAO group had significant differences when compared with the sham-operation group （P〈0. 01）, the CCF group had significant differenees compared with the MCAO group （P（0. 01）. Conclusions CCF tablets can predominantly enhance the expression of PSA-NCAM in the brain of MCAO rats, might to promote the neurogenesis anti-apoptosis in acute ischemia cerebral disease.

关 键 词：中药 脑缺血 多唾液酸一神经细胞黏附分子 

分 类 号：R743.1[医药卫生—神经病学与精神病学]