Effects of culture media and three metal ions on the accumulation of lanosterol and ergosterol in cultured mycelia of Inonotus obliquus  

作　　者：ZHENG Wei-Fa XIANG Xiao-Yan CHEN Cai-Fa WANG Yao ZHAO Yan-Xia JIANG Ji-Hong CHU Cheng-Cai 

机构地区：[1]Key Laboratory for Biotechnology on Medicinal Plants of Jiangsu Province, Xuzhou Normal University, Xuzhou 221116, China [2]School of Biological Sciences, Anqing Normal College, Anqing 26400, China [3]Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China

出　　处：《菌物学报》2008年第1期126-139,共14页Mycosystema

基　　金：Supported by Natural Science Foundation of the Education Department of Jiangsu Province （No. 05KJA36012）, and Natural Science Foundation of Jiangsu Province （BK2006034）

摘　　要：Lanosterol and ergosterol are the active principles with potential pharmacological activities in Inonotus obliquus. However, the two sterols are less accumulated in cultured mycelia of the fungus. In this study, different carbon and nitrogen sources and pH levels together with three metal ions were assayed for their effects on accumulation of the two sterols in the fungus. Among the tested media the growth medium consisting of glucose (1.5%), rice powder (0.5%), yeast extract (0.4%), wheat bran (0.1%), KH2PO4 (0.01%) and MgSO4·7H2O (0.05%) with pH level at 6.5 yielded a maximum production of the two sterols, which can further be increased following the treatment of Ag+, Cu2+ and Ca2+. Supplementing Ag+ at concentrations of 0.28 and 0.35μmol partially inhibited ergosterol biosynthesis, leading to an enhanced accumulation of lanosterol, the presence of intermediates of ergosterol biosynthetic pathway and a reduced accumulation of ergosterol in cultured mycelia of I.Lanosterol and ergosterol are the active principles with potential pharmacological activities in Inonotus obliquus. However, the two sterols are less accumulated in cultured mycelia of the fungus. In this study, different carbon and nitrogen sources and pH levels together with three metal ions were assayed for their effects on accumulation of the two sterols in the fungus. Among the tested media the growth medium consisting of glucose （1.5%）, rice powder （0.5%）, yeast extract （0.4%）, wheat bran （0.1%）, KH2PO4 （0.01%） and MgSO4·7H2O （0.05%） with pH level at 6.5 yielded a maximum production of the two sterols, which can further be increased following the treatment of Ag^＋, Cu^2＋ and Ca^2＋. Supplementing Ag^＋ at concentrations of 0.28 and 0.35μmol partially inhibited ergosterol biosynthesis, leading to an enhanced accumulation of lanosterol, the presence of intermediates of ergosterol biosynthetic pathway and a reduced accumulation of ergosterol in cultured mycelia of I. obliquus.

关 键 词：培养基 金属离子 桦褐孔菌 菌丝体 羊毛甾醇 麦角甾醇 积累 

分 类 号：S646[农业科学—蔬菜学]