病原性溶藻弧菌对大黄鱼鳃黏液黏附作用研究  

Adhesion of pathogenic Vibrio alginolyticus to the gill mucus of Pseudosciaena crocea

作　　者：鄢庆枇[1] 陈强[1] 马甡[2] 庄峙厦[3] 王小如[3] 

机构地区：[1]集美大学水产学院,福建厦门361021 [2]中国海洋大学水产学院,山东青岛266003 [3]国家海洋局第一海洋研究所,山东青岛266061

出　　处：《海洋学报》2007年第6期80-86,共7页

基　　金：国家863计划项目(2002AA639600);福建省科技项目(2006F5066);福建省高校水产科学技术与食品安全重点实验室开放基金(2007J101)

摘　　要：采用^3H-TdR同位素示踪方法研究了溶藻弧菌对大黄鱼鳃黏液黏附作用.结果表明,溶藻弧菌的黏附量随着菌浓度的增加而升高并在180 min后达到饱和;在温度15～30℃、pH偏酸条件下黏附作用较强,Na＋是溶藻弧菌黏附作用必需的,Ca^2＋起着辅助作用;溶藻弧菌经营养饥饿、热处理及高碘酸处理后黏附作用有明显下降;8种碳水化合物都有不同程度的促进溶藻弧菌的黏附作用,其中葡萄糖、甘露糖、果糖及麦芽糖有明显促进作用.研究结果表明：海水中的溶藻弧菌可很好地黏附于大黄鱼鳃黏液,其黏附作用受环境因子影响,并且与溶藻弧菌的表面碳水化合物、热敏结构等有着密切关系.Adhesion of Vibrio alginolyticus to the gill mucus of Pseudosciaena crocea had been investigated using [methyl-^3H]thymidine as isotope tracer. The results showed that： the adhesive quantity of V. alginolyticus increased with bacterial concentrations and reached equilibrium after incubated for 180 mini thehigher adhesive quantity was obtained at 15～30℃ and sourish conditions; adhesion of V. alginolyticus could not achieved without Na^＋ , and Ca^2＋ played an auxiliary role in bacterial adhesion; adhesion of V. alginolyticus was inhibited remarkably by starvation, heat treatment and periodic acid treatment; all of the 8 kinds of carbohydrates investigated enhanced the adhesion of V. alginolyticus to the gill mucus of P. crocea, among them, glucose, mannose, fructose and maltose showed the specially enhanced adhesion. The results indicated that V. alginolyticus could adhere to the gill mucus of P. crocea facilely in seawater, this bacterial adhesion was influenced by environmental factors and closely relation with superficial carbohydrate structures and some heat-sensitive structures.

关 键 词：溶藻弧菌 黏附作用 大黄鱼 鳃黏液 

分 类 号：Q949.321[生物学—植物学] S965.131[农业科学—水产养殖]