丹玄口康对ANE诱导人口腔黏膜FB胶原合成及TGFβ_1表达的抑制作用  

Inhibition of Danxuan Koukang on collagen synthesis and TGFβ_1 expression in human oral mucosal fibroblasts induced by areca nut extract

作　　者：李元聪[1] 谭劲[1] 陈安[2] 彭楚湘[2] 伍春华[1] 

机构地区：[1]湖南中医药大学第一附属医院,湖南长沙410007 [2]湖南中医药大学,湖南长沙410007

出　　处：《湖南中医药大学学报》2007年第6期15-17,共3页Journal of Hunan University of Chinese Medicine

基　　金：国家自然科学基金项目(30472231)

摘　　要：目的研究丹玄口康(Danxuan Koukang,DXKK)含药血清对槟榔提取物(Areca Nut Extract,ANE)诱导人口腔黏膜成纤维细胞(Fibroblasts,FB)的胶原合成及β1转化生长因子(transforming growth factorβ1,TGFβ1)表达的影响。方法体外培养人口腔黏膜FB,ANE为诱导剂,10%大鼠丹玄口康药物血清为干预物;用3H-脯氨酸掺入法检测胶原含量,用免疫细胞化学法和ELISA法检测TGFβ1的表达。结果3H-脯氨酸掺入结果显示,ANE对人口腔黏膜FB胶原合成有促进作用,DXKK含药血清对ANE刺激下的FB胶原合成有抑制作用(P<0.05或P<0.01),并呈剂量-效应关系;免疫细胞化学和ELISA结果显示,丹玄口康含药血清对ANE所致FB的TGFβ1表达有抑制作用(P<0.01)。结论丹玄口康含药血清可通过下调TGFβ1因子的表达,而拮抗ANE导致的胶原合成。Objective To study the effect of prepared Danxuan Koukang （DXKK） drug serum on collagen synthesis and expression of transcausing growth factor β1 （TGF31） in human oral mucosal fibroblasts（FB） induced by areca nut extract（ANE）. Methods Human oral mucosal FB were cultured in vitro with inducement ANE and intervenor 10% DXKK drug serum; Collagen synthesiswas measured by ^3H-proline; and TGFβ1 expression was examined by immunocytechemistry and ELISA. Results ^3H-proline incorporation value showed that ANE could promote collagen synthesis of human oral mucosal FB, the promotion was restrained by DXKK drug serum in a manner of dose-effect relationship （P〈0.01 or P〈0.05）; immunocytechemistry arid ELISA showed that the TGFβ1 expression of human oral mucosal FB was depressed by DXKK drug serum （P〈0.01）. Conclusions DXKK drug serum can inhibit the collagen synthesis stimulated by ANE through down-regulating TGFβ1 expression.

关 键 词：丹玄口康 槟榔提取液 成纤维细胞 胶原 转化生长因子β1 大鼠 

分 类 号：R285.5[医药卫生—中药学] R276.815[医药卫生—中医学]