短暂性脑缺血发作与晚期糖基化终末产物受体基因Gly82Ser多态的关联  

Gene Gly82Ser polymorphism of receptor of advanced glycaton end products in patients with transient ischemia attack

作　　者：王海萍[1] 赵仁亮[1] 宋晓瑾[2] 张晨[1] 滕继军[1] 宋玉强[1] 

机构地区：[1]青岛大学医学院附属医院神经内科,266003 [2]青岛市第八人民医院儿科

出　　处：《中华神经科杂志》2007年第12期827-831,共5页Chinese Journal of Neurology

摘　　要：目的探讨晚期糖基化终末产物受体(receptor of advanced glycaton end products,RAGE)基因 Gly82Ser 多态与短暂性脑缺血发作(TTA)的关系。方法 130例 TIA 患者按是否合并糖尿病分为单纯性 TIA 组60例,TIA 合并糖尿病组(糖尿病&TIA)70例,健康对照组66名,应用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)方法检测 RAGE 基因 Gly82Ser 外显子3内82位点基因型,比较各组基因型频率和等位基因频率。结果共检测到3种基因型 GG、Gs 及 SS,糖尿病&TIA、单纯 TIA 及健康对照3组患者 RAGE 基因杂合子基因型(GS)频率分别为62.9%、53.3%和43.9%,糖尿病&TIA 组明显高于对照组(OR 2.036,95% CI 1.021～4.062,P=0.042);而单纯 TIA 组与对照组之间差异无统计学意义(OR 1.299,95% CI 0.644～2.618,P=0.465);3组等位基因 S 频率分别为34.3%、28.3%和26.5%,3组之间差异均无统计学意义。结论 TIA&糖尿病患者 RAGE 基因Gly82Ser 基因型(GS)增多,Gly82Ser 的多态可能是糖尿病&TIA 患者的危险因素,未发现其与单纯性TIA 有关。Objective To explore the relationship between receptor of advanced glycaton end products （RAGE） gene Gly82Ser polymorphism and patients with transient ischemia attack（TIA）. Methods The Gly82Ser gene at the position of RAGE gene exon 3 was identified by a polymerase chain reaction- restriction fragment length polymorphism （PCR-RFLP） method in 70 cases of TIA ＆ Diabetes（ DM）, 60 of simply TIA and 66 healthy control subjects. Results The genotypes of RAGE gene Gly82Ser identified were GG, GS and SS. The frequencies of RAGE gene Gly82Ser GS heterozygous genotype of TIA ＆ DM and control were respectively 62. 9% and 43. 9%, significantly higher in TIA ＆ DM patients than in control subjects （ OR 2. 036, 95% CI 1. 021-4. 062, P = 0. 042）, however no significant difference was found between simply TIA and control （ 53. 3% vs 43.9%, OR 1. 299,95% CI 0. 644-2. 618, P = 0. 465 ）. Significant difference of the frequency of S allele was found neither between TIA ＆ control and control （ being 34. 3% and 26. 5%, respectively, OR 1. 446,95% CI 0. 859-2. 434, P = 0. 164）, nor between simple TIA andcontrol （28.3% vs26.5%, OR 1.096,95%CI0.630-1.907, P=0.746）. Conclusions RAGE gene Gly82Ser GS heterozygous genotype may be associated with TIA ＆ DM patients. RAGE gene Gly82Ser polymorphism is a risky factor for TIA ＆ DM patients, but not for TIA patients.

关 键 词：脑缺血发作 短暂性 糖尿病 受体 免疫 多态性 限制性片段长度 

分 类 号：R743.3[医药卫生—神经病学与精神病学]