PCR-SSCP分析法检测宫颈癌中H-ras、p^(53)基因突变与HPV感染  

Detection of p^(53) H-ras Gene Mutations by PCR-SSCP analysis and Human Papillomavirus Infection in Cervical Carcinoma

作　　者：邱小萍[1] 伍欣星[1] 丁晓华[1] 谭云[1] 赵文先[1] 戴天力 

机构地区：[1]湖北医科大学病毒研究所,武汉430071

出　　处：《肿瘤防治研究》1997年第4期222-224,共3页Cancer Research on Prevention and Treatment

基　　金：湖北科委八.五课题基金

摘　　要：我们对40例宫颈癌活检组织同时进行HPV、H－ras、p53基因的检测，应用GP－PCR技术检测了多型HPV的感染，发现阳性率为85％（34／40）。HPV16转化基因E7占61．8％（21／34）。此外，利用PCR－SSCP（单纯DNA构象多态性）分析方法，对宫颈癌中H－ras、p53基因点突变进行了检测，H－ras突变占22．5％（9／40），p53占2．5％（1／40），而H－ras突变在HPV感染阳性组织中占14．7％（5／34），其中3例的病程分期为Ⅲ期以上。结果表明：HPV16E7转化基因在宫颈癌的致癌过程中为关键基因，H－ras突变很可能与有关报道相一致，属于“迟发”事件，与肿瘤的恶性程度及浸润有关。而p535—6外显子基因突变在HPV感染的宫颈癌中发生率很低。p53、H-ras gene mutations and human papillomavirus infection were detected in 40 spedments of cervical carcinoma. GP-PCR (general primers-PCR) was applied to testing for various HPV infection. The positive rate was 85% (34/40). The incidence of HPV16 E7 transformed gene was 61. 8% (21/34). Otherwise, H-ras、p53 gene mutation were detected by PCR-SSCP analysis (Single strand con formation polymorphism analysis of polymerase chain reaction products). Mutation rates were respectively 22. 5% (9/40) and 2. 5 % (1/40). H-ras mutation was 14. 7% (5/34) in 34 HPV-positive, biopsy tissues. The results show: HPV16 E7 transformed gene is a critical gene in the develepment of cervical carcinoma. Mutation of H-ras gene is infrequent in cervical carcinoma. But it is possible to play an important roly in latter stages. p53 mutation is infrequent in cervial carcinomal with HPV infection.

关 键 词：P53 转化基因E7 子宫颈癌 乳头状瘤病毒 

分 类 号：R737.330.3[医药卫生—肿瘤] R730.231.3[医药卫生—临床医学]