组织芯片和常规免疫组化法检测软组织肉瘤标志物的比较  

Comparative analysis on the expression of tumor marker in soft tissue sarcoma by tissue microarray and by immunohistochemistry

作　　者：张学军[1] 丛琦[1] 郝彦勇[1] 王志生[1] 王帅[1] 闫光志[1] 

机构地区：[1]吉林省肿瘤医院,吉林长春130012

出　　处：《吉林医学》2007年第17期1827-1828,共2页Jilin Medical Journal

摘　　要：目的:通过石蜡组织芯片和免疫组织化学检测软组织肉瘤标志物的对比,确定其临床应用的可靠性。方法:选取80例软组织肉瘤标本,采用组织微阵列技术,构建8×10阵列,光镜观察和计数Ki-67、P53、PCNA在软组织肉瘤组织中的表达情况及对应常规免疫组化方法检测结果。结果:组织芯片和免疫组化的检测结果是一致的,两者比较差异无显著性,P>0.05。结论:石蜡组织芯片和常规免疫组化检测Ki-67、P53、PCNA的结果完全一致,应用组织芯片检测肿瘤标志物可以替代常规免疫组化的检查。Objective To examine the reliability of tissue microarrary （TMA） for detecting tumor marker in soft tissue sarcoma compared to the immunohistochemistry. Method Tissue microarray technology was used to detect the expression of Ki-67 ,PCNA and P53 in 80 cases of STS and compared the results with the immunohistochemistry. Results No difference was found between the immunohistochemistry results by 1.5 mm tissue microarray and the negligible difference was found between by traditional pathological technology and by tissue microarray in soft tissue sarcoma. Conclusion On the positive rate of Ki-67, PCNA and p53, no significant difference is found be- tween the results with the traditional pathological investigation and with the tissue microarray （ P 〉 0.05 ）. It has important practical significance and broad application prospect in pathology.

关 键 词：软组织肉瘤 组织芯片 免疫组化 肿瘤标志物 

分 类 号：R738.6[医药卫生—肿瘤]