DAC对卵巢癌细胞株增殖及其MAGE、BAGE、GAGE基因表达的影响  

Effect of 5-aza-2′-deoxycytidine in proliferation of ovarian cancer cell lines and its effects on expression of MAGE,BAGE,GAGE gene

作　　者：韩风贤 张师前[2] 周晓亮[2] 吴喜梅[2] 石琰[2] 

机构地区：[1]海口市妇幼保健院,海南海口570216 [2]山东大学齐鲁医院

出　　处：《山东医药》2007年第36期33-35,共3页Shandong Medical Journal

基　　金：山东省优秀中青年科研基金资助项目(2005BS03013)

摘　　要：目的探讨5-杂氮脱氧胞嘧啶(DAC)对卵巢癌细胞株SKOV3、A2780、COC1增殖及其肿瘤特异性抗原MAGE、BAGE、GAGE基因表达的影响。方法将对数生长期的卵巢癌细胞株与DAC共同培养,观察细胞生长指数,利用流式细胞术检测细胞周期,RT-PCR技术检测卵巢癌细胞株在DAC作用前后MAGE、BAGE、GAGE基因的表达。结果DAC能使大多数巢癌细胞株阻滞在G0期,并使其MAGE、BAGE、GAGE基因表达增加。结论DAC能抑制卵巢癌细胞株的增殖,提高其肿瘤特异性抗原基因的表达。[Objective] To investigate the effect of 5-aza-2＇-deoxyeytidine （DAC）in proliferation of ovarian cancer cell lines and expression of tumor specific antigen MAGE, BAGE and GAGE. [ Methods ] Ovarian cancer cell lines were treated with DAC in co-culture, the growth curves were observed, the cell cycle was detected by flow cytometry, the MAGE, BAGE, GAGE gene expression patterns were measured by RT-PCR before and after DAC treatment. [ Results ] The growth of most cells were stopped in GO phases after DAC treatment,the levels of MAGE, BAGE,GAGE gene expressions after DAC treatment were higher than before. [ Conclusion ] DAC has an inhibitive effect on the ovarian cancer cell lines, and can elevate the expression of tumor specific antigen gene .

关 键 词：5-杂氮脱氧胞嘧啶 卵巢肿瘤 肿瘤特异性抗原基因 

分 类 号：R737.31[医药卫生—肿瘤]