B7-1(CD80)基因的克隆及其在小鼠黑色素瘤细胞中的表达  

THE CLONING OF B7 1 GENE AND ITS EXPRESSION IN MOU SE MELANOMA CELLS

作　　者：隋拥君[1] 丁广治[1] 唐佩弦[1] 侯春梅[1] 毛宁[1] 

机构地区：[1]军事医学科学院基础医学研究所

出　　处：《免疫学杂志》1997年第3期164-167,共4页Immunological Journal

摘　　要：应用ＲＴ－ＰＣＲ技术从人Ｂ淋巴瘤细胞系Ｒａｊｉ中克隆到Ｂ７－１（ＣＤ８０）ｃＤＮＡ，并经测序证实。将其插入至真核表达载体ｐＬＸＳＮ中，用脂质体转染小鼠黑色素瘤细胞Ｂ１６，Ｇ４１８筛选，并经流式细胞仪检测，得到了细胞表面表达Ｂ７－１的重组克隆。ＰＣＲ从其基因组ＤＮＡ中扩增到Ｂ７－１基因，提示Ｂ７－１ｃＤＮＡ已整合至宿主细胞的基因组ＤＮＡ中。本文为研究Ｂ７－１在肿瘤免疫中所起的作用奠定了基础。B7 1(CD80) cDNA was cloned by RT PCR from human B lymphma Raji cell line and confirmed by DNA sequencing.The recombinant expressing vector was constructed by inserting the B7 1 cDNA into pLXSN vector.After transfecting by lipofectamine and selecting with G418,the B7 1 transfected recombinant clone was obtained.It was confirmed that the B7 1 cDNA had been integrated into the genomic DNA of B16 B7 cells by PCR analysis.Our study enables us to further investigate the role of B7 1 in tumor immunotherapy.

关 键 词：B7-1(CD80) 基因克隆 基因表达 黑色素瘤 

分 类 号：R73－35[医药卫生—肿瘤] Q785[医药卫生—临床医学]