亚低温对脑出血后凝血酶受体Ⅰ表达影响的实验研究  

The effect of mild hypothermia on expression of thrombin receptor-1 following intracerebral hemorrhage in rats

作　　者：李晓玲[1] 石正洪[1] 张振昶[1] 卫景沛[1] 

机构地区：[1]兰州大学第二医院神经内科,甘肃兰州730030

出　　处：《中风与神经疾病杂志》2007年第6期700-702,共3页Journal of Apoplexy and Nervous Diseases

摘　　要：目的观察亚低温对大鼠自体血注入法脑出血模型凝血酶受体(PAR-1)表达的影响,探讨亚低温减轻脑出血后脑水肿的可能机制。方法雄性SD大鼠108只,随机分为生理盐水组、脑出血组和脑出血+亚低温组。每组分为脑出血后24h、72h和7d共3个亚组。脑出血+亚低温组于注血后给予6h亚低温治疗。各组于注血后24h、72h和7d断头取脑组织,测定脑水含量(干湿重法),检测脑内血肿周围微血管壁上PAR-1的表达(免疫组化学法)。结果脑出血组血肿周围微血管壁上PAR-1的表达高于生理盐水组(P<0.05)。亚低温组血肿周围微血管壁上PAR-1的表达低于脑出血组(P<0.01)。同时,亚低温组脑水含量在各时间点与脑出血组比较明显下降(P<0.01)。结论脑出血后血肿周围微血管壁上PAR-1的表达增加,其变化与脑水肿的变化一致,说明PAR-1参与了脑出血后脑水肿的形成;亚低温可能通过抑制出血后微血管壁上PAR-1的表达来发挥其脑保护作用。Objective To examine the effect of mild hypothermia on the expression of PAR-1 following intracerebral hemorrhage in rats and clarified the mechanism of hypothermia on edema formation following ICH. Method One hundered and eight male rats were randomly divided into three groups：the normal saline group,the cerebral hemorrhage group,in which autologous arterial blood were stereotaxically injected into right caudate nucleus,and the mild hypothermia group,in which the rats were given mild hypothemia after the injection of blood. Each group was divided into 3 subgroups ： 24h, 72h and 7d after operation. Dry-wet weight was used to measure brain water content. Meanwhile,immunostaining was used to detect the PAR-1 expression. Result In cerebral hemorrhage, the PAR-1 expression in tissue surrounding hemotoma were markedly increased at 24h after intracerebral hemorrhage,peaked at 72h and decreased at 7d after intracerebral hemorrhage. In mild hypothermia group, compared with intracerebral hemorrhage,both the elevation of PAR-1 expression and brain water content was attenuated at all time point by hypothermia. Conclusions PAR-1 expression is upregulated during ICH. Mild hy- pothermia can suppress the expressing of PAR-1.

关 键 词：亚低温 脑出血 凝血酶受体 脑水肿 

分 类 号：R743.3[医药卫生—神经病学与精神病学]