牙槽骨缺损与弹性结扎快速构建慢性牙周炎Beagle犬模型的研究  

The Study of Rapidly Construction of Chronic Periodontitis in Beagle Dogs Caused by Alveolar Bone Defect with Ligation

作　　者：董妮[1] 宋锦璘[1] 冯格[1] 刘珉懿[1] 杜永洪[2] 赵纯亮[2] 王智彪[2] 邓锋[1] 

机构地区：[1]重庆医科大学附属口腔医院,重庆400015 [2]重庆医科大学医学超声工程研究所,重庆400016

出　　处：《西南师范大学学报（自然科学版）》2008年第1期62-66,共5页Journal of Southwest China Normal University(Natural Science Edition)

基　　金：超声医疗国家工程研究中心资助;国家教育部创新团队发展计划资助项目(教技函[2005]33号)

摘　　要：探索快速构建牙槽骨缺损联合弹性结扎致慢性牙周炎Beagle犬模型的方法,为超声治疗慢性牙周炎提供研究平台.6只Beagle犬选择上下颌双侧第三前磨牙颊侧翻瓣,制备6 mm×6 mm×3 mm的牙槽骨缺损,左右侧采用自身对照.对照组直接缝合牙龈,实验组缝合牙龈后一周置入正畸弹性牵引橡皮圈,术后第2,4,6,8,10周摄X片,行形态学和组织学观察.临床检查以牙龈发红、水肿、探诊出血、牙周袋形成为主要症状.第10周左右,牙龈炎逐渐向牙周炎发展,观察到牙周袋形成、牙槽骨吸收、以及显著的病理性成骨.牙槽骨缺损联合弹性结扎可快速构建牙周炎模型,缩短慢性牙周炎Beagle犬模型构建周期,较好地模拟临床牙周炎的病理病变过程,值得推广应用.The authors study the fast way to establish the Beagle dog model of chronic periodontitis caused by alveolar bone defect with ligation, to provide the research platform for the further approach of the ultrasonic therapy of periodontitis. All the third premolars of six Beagle dogs were removed alveolar bone （6×6×3 mm^3） by surgical way. Left and right sides were divided into the experimental group and the control group. On one side, flaps of the jaws were sutured immediately. The other side were sutured and the or- thodontic elastic ligation were placed after one week. The Beagle dogs were observed through periodontal clinical index and X-ray examinations of experimental tooth in 2^rd, 4^th, 6^th, 8^th and 10^th weeks. Morphology and Histological studies were performed after 10^th weeks. Clinical examination found that gingival redness, edema, bleeding on probing, pocket formation were the main symptoms. As time progressed, the symptoms gradually changed from gingivitis to periodontitis. The periodontal pocket formation and alveolar bone absorption appeared. Those results indicated that rapidly construction of periodontitis model can be produced by combination of alveolar bone defect with ligation, which simulated the clinical pathological periodontal disease process fastly. It could provide a better way for rapid construction of chronic periodontitis in Beagle dog.

关 键 词：慢性牙周炎 BEAGLE犬 快速 

分 类 号：Q95[生物学—动物学] R781.4[医药卫生—口腔医学]