炎症性肠病患者外周血CD4^＋CD25^＋Treg细胞及其特异标志物Foxp3表达水平的研究  

Study on expression of CD4^+CD25^+ T regulatory cell and its specific markers Foxp3 in peripheral blood of inflammatory bowel disease patients

作　　者：孙可歆[1] 李永哲[2] 赵臣[1] 刘定华[2] 胡朝军[2] 佟大伟[2] 张蜀澜[2] 

机构地区：[1]吉林医药学院检验系,吉林132013 [2]中国医学科学院中国协和医科大学北京协和医院检验科,北京100730

出　　处：《中国免疫学杂志》2008年第1期71-74,共4页Chinese Journal of Immunology

基　　金：国家自然科学基金资助项目(No.30471617);国家高技术研究发展技划(863计划)重大专项基金资助项目(No.2002AARZ2011)

摘　　要：目的:通过检测炎症性肠病(IBD)不同病期患者以及对照组外周血CD4+CD25+Treg及其特异标志物Foxp3的表达,来分析与IBD疾病活动性关系,探讨CD4+CD25+Treg和Foxp3在IBD发病机制中的作用。方法:52例IBD患者和35例正常对照组分别应用流式细胞术和逆转录-聚合酶链反应(RT-PCR)检测外周血中CD4+CD25+T细胞亚群的百分率测定和外周血单个核细胞Foxp3mRNA的表达水平。结果:IBD患者外周血CD4+CD25+Treg细胞比例明显低于疾病缓解期患者和正常对照组(P<0.01);活动期IBD患者中使用激素和/或免疫抑制剂与未使用激素和/或免疫抑制剂结果差异有统计学意义;IBD患者外周血单个核细胞(PBMC)中Foxp3 mRNA表达水平低于缓解期和正常人,差异有显著性(P<0.05);缓解期Foxp3 mRNA水平与正常人差异无显著性(P>0.05);IBD患者外周血CD4+CD25+Treg细胞表达率及PBMC中的Foxp3 mR-NA表达水平与疾病活动指数评分呈负相关性。结论:活动期IBD患者外周血CD4+CD25+Treg细胞及Foxp3 mRNA表达下调,而恢复期其表达回升,且二者呈正相关,并与临床活动评分呈负相关,因此认为CD4+CD25+Treg细胞和Foxp3可能参与疾病的发生发展,与疾病的活动性密切相关。Objective：To investigate the level ofCD4^＋CD25^＋T cell and the Foxp3 in peripheral blood of the inflammatory bowel disease（ IBD）, and analyzs the relationship between its pathogenesis and activity. Methods：The peripheral blood CD4^＋CD25^＋T lymphoeytes frequencies and Foxp3 mRNA expression on peripheral Blood monoeytes of 52 IBD patients and 35 normal controls were used to there-color FACs analysis and Reverse transeription-polymerase chain reaction （RT-PCR）. Results ： The expression of CD4^＋CD25^＋T cells in active group IBD patients is significantly lower than remission group and healthy eontrols（P 〈 0. 01 ） ;The difference of the results between active group patients who are taking and never take glueoeortieoids and immunosuppressive drugs have significantly meaning in statistics ;Foxp3 mRNA expression of IBD in active stage was lower than that of controls （P 〈 0. 05 ）, while in remission stage there was no difference with that of controls （P 〉 0.05 ） ;The relationship between peripheral blood CD4^＋CD25^＋Treg cell ,the Foxp3 mRNA and active renal score shows negative correlation. Conclusion：The expression of peripheral blood CD4^＋CD25^＋Treg cell and the Foxp3 mRNA is abnormal and it maybe correlated to pathogenesis and activity of IBD.

关 键 词：炎症性肠病 外周血单个核细胞 CD4^+CD25^+T调节细胞 FOXP3 

分 类 号：R574.62[医药卫生—消化系统]