异基因移植患者进行Id4基因检测的意义初探  

Study on Id4 Gene Methylation in Acute Leukemia Patients undergoing allo-PBSCT

作　　者：赵瑜[1] 靖彧[1] 薄剑[1] 李红华[1] 孙敬芬[1] 高春记[1] 于力[1] 

机构地区：[1]中国人民解放军总医院血液科,北京100853

出　　处：《医学临床研究》2007年第12期2017-2018,共2页Journal of Clinical Research

基　　金：973国家重点基础研究专项经费(2005CB522400)

摘　　要：【目的】探讨在行异基因外周血干细胞移植的急性白血病(AL)患者中进行Id4基因甲基化检测对预测临床复发意义。【方法】用甲基化特异性PCR(MS-PCR)方法对16例接受异基因外周血干细胞移植的AL患者,检测其移植前、移植后6个月和12个月的骨髓Id4基因甲基化状态,并随诊分析。【结果】10例患者移植前Id4基因呈甲基化的患者中5例在移植后再次出现Id4基因甲基化,其中4例在随访期出现复发或复发倾向。而5例移植前Id4基因呈非甲基化的患者中,无一例在移植后再次甲基化,随访期间也无复发倾向。【结论】AL患者在移植后进行Id4基因甲基化检测对于预测白血病的复发可能有一定意义。[Objective] To study the significance of Id4 gene methylation in acute leukemia （AL） patients undergoing allo-PBSCT. [Methods] Id4 gene methylation was detected by methylation specific-polymerase chain reaction （MS-PCR） in 18 AL patients before and 6 and 12 months after allo-PBSCT. [Results] Among 10 patients with Id4 gene methylation before allo-PBSCT, 5 cases were found Id4 methylation after allo-PBSCT and 4 cases of them were found relapse or relapse tendency in the coming 6- 12 months, whereas all the 5 patients with Id4 gene unmethylation before allo-PBSCT were still at their Id4 non-methylation and complete remission status after allo-PBSCT. [Conclusion] Detecting the status of Id4 gene methylation in AL patients after allo-PBSCT might have clinical significance on forecasting AL relapse.

关 键 词：造血干细胞移植 基因/分析 

分 类 号：R394.2[医药卫生—医学遗传学]