荷S-180移植瘤小鼠血清干扰素γ含量的变化及免疫调节药物的干预作用  

Changes of serum interferon-γ levels in mice bearing S-180 tumor and the interventional effect of immunomodulators

作　　者：李丽[1] 雷林生[1] 余传林[1] 朱正光[1] 张群[1] 李建军[2] 陈娜娜[1] 吴曙光[1] 

机构地区：[1]南方医科大学药学院药物研究所,广东广州510515 [2]南方医科大学基础医学院,广东广州510515

出　　处：《南方医科大学学报》2008年第1期65-68,共4页Journal of Southern Medical University

基　　金：广东省自然科学基金(04020361)~~

摘　　要：目的研究荷S-180肉瘤小鼠血清干扰素γ(IFN-γ)含量的变化,并采用具有调节免疫功能的药物进行干预,探讨内源性IFN-γ在抗小鼠移植瘤方面的意义。方法建立荷S-180实体瘤小鼠动物模型,分别用灵芝多糖(GLP)、环孢素A(CsA)灌胃给药,每天1次,连续9d,用ELISA试剂盒检测小鼠血清中IFN-γ的含量,并分离肿瘤称重。结果首次发现荷S-180实体瘤小鼠血清中IFN-γ含量在所观察的接种后20d内随荷瘤时间的延长而升高。GLP(400、200、100mg/kg)使荷瘤小鼠血清中的IFN-γ含量增加、肿瘤重量减轻,但增加IFN-γ含量以高剂量最为明显,抑瘤效应以中剂量最佳,两者之间相关关系无统计学意义。CsA(20、10、5mg/kg)可降低荷瘤小鼠血清中IFN-γ的含量,但对肿瘤重量无明显影响,两者之间无相关关系。结论灵芝多糖增加荷瘤小鼠血清中IFN-γ含量与抑瘤效应无明显相关,环孢素A降低荷瘤小鼠血清中IFN-γ的含量对肿瘤重量无明显影响,提示内源性IFN-γ在抗小鼠S-180移植瘤方面并不是主要的免疫调节因素。Objective To investigate the changes of serum inteferon-γ （IFN-γ） in mice bearing S- 180 tumor and explore the role of the endogenous IFN-γ in confining the transplanted tumor by intervention with immunomodulators. Methods Mouse models bearing S-180 solid tumor were established and subjected to intragastric administration of Ganoderma lucidum polysaccharides （GLP） or cyclosporine A （CsA） at different daily doses for 9 consecutive days. Serum IFN-γ levels were measured in untreated tumor-bearing mice and in those after completion of GLP or CsA treatments by enzyme-linked immunosorbent assay （ELISA）, and the changes of the tumor weight in the treated mice were evaluated. Results It was found for the first time that serum IFN-γ levels in the tumor-bearing mice increased progressively within the initial 20 days after tumor implantation. The serum IFN-γ levels in the 3 GLP-treated groups （at daily doses of 400, 200, and 100 mg/kg） all increased, which was the most obvious in 400 mg/kg GLP-treated group, and the tumor weight decreased significantly in response to GLP treatment, but the most conspicuous effect occurred with the daily dose of 200 mg/kg, and no significant statistical correlation was found between the two parameters. CsA treatment （at 20, 10, and 5 mg/kg, respectively） resulted in reduced serum IFN-γ levels but produced virtually no effect on the tumor weight, and no obvious correlation was found between serum IFN-γ level and the tumor weight. Conclusion Increased serum IFN-γ levels following GLP treatment are not significantly correlated to tumor growth inhibition in mice, and CsA reduces serum IFN-γ levels without affecting the tumor weight, suggesting that endogenous IFN-γ is not a major immunomodulating factor in growth inhibition of transplanted S-180 tumor.

关 键 词：肿瘤 S-1 80 干扰素Γ 灵芝多糖 环孢素A 

分 类 号：R730.5[医药卫生—肿瘤]