阿霉素对人胃癌细胞GSTπ表达的影响  

Effects of Adriamycin on the Expression of GSTπ in Human Gastric Carcinoma Cells

作　　者：蒋丽娜[1] 张从胜[1] 齐效勇[1] 薄爱华[2] 张晓丽[2] 

机构地区：[1]河北北方学院医学技术学院免疫教研室,河北张家口075000 [2]河北北方学院实验中心电镜室,河北张家口075000

出　　处：《河北北方学院学报（医学版）》2008年第1期25-26,共2页Journal of Hebei North University:Medical Edition

摘　　要：目的:探讨阿霉素对人胃癌细胞的杀伤作用及致GSTπ表达的变化。方法:利用人胃癌BGC-823细胞株,CO2培养箱进行培养过程中,加入阿霉素注射液继续培养5h或10h。然后用HE和免疫组化染色。光镜下观察并进行图象分析。结果:实验组BGC-823细胞变形,GSTπ表达位置和灰度有明显变化。结论:阿霉素作用5h可对胃癌细胞有杀伤作用,使胃癌细胞形态和GST基因表达有明显变化。Objective：To study the killing function of Adriamycin to human gastric carcinoma ceils and its influence to the expression of GSTπ. Methods： While BGC-823 gastric carcinoma cells were educated in CO2 training box, Adriamycin was injected in human gastric carcinomal cells to educate continuously for 5h or 10h, then stained with Haematoxylin-Eosinc（ HE） and dyed with immunohistochemical method. The result was observed and analyzed with optical microscope. Results： BGC-823 cells was deformed in experimental group, and expressive location and ash degree of GSTπ had obvious changes. Conclusion： Adriamycin has killing function on carcinoma cells and it can make Gastric carcinoma cells deformed and change the expression of GSTπ.

关 键 词：阿霉素 胃肿瘤/细胞 谷胱甘肽S转移酶Π 免疫组织化学 

分 类 号：R735.2[医药卫生—肿瘤]