p21活化激酶4在胃癌中的表达及意义  

Expression of PAK4 and its significance in gastric cancer

作　　者：李泽[1] 徐惠绵[1] 李丰[2] 

机构地区：[1]中国医科大学第一附属医院肿瘤科,沈阳110001 [2]中国医科大学细胞生物学卫生部重点实验室

出　　处：《中华肿瘤杂志》2008年第1期30-33,共4页Chinese Journal of Oncology

基　　金：国家自然科学基金资助项目（30370640、30370736）

摘　　要：目的探讨p21活化激酶4（PAK4）在人胃癌组织和胃癌细胞系中的表达及与临床病理因素的关系。方法免疫组化法检测95例人胃癌组织中PAK4蛋白的表达；Westernblot法检测40例新鲜胃癌组织和相应的癌旁正常胃黏膜组织以及7种人胃癌细胞系中PAK4蛋白的表达。结果95例人胃癌组织中，PAK4蛋白的阳性表达率为67．4％（64／95），其中62例（96．9％）阳性染色位于细胞浆中，仅有2例（3．1％）位于细胞膜上。PAK4蛋白的阳性表达率与胃癌TNM分期（Χ^2=10．426，P〈0．01）和淋巴结转移（Χ^2=4．912，P〈0．05）有关。新鲜胃癌组织中PAK4蛋白的表达量显著高于癌旁正常胃黏膜组织（t=-5．978，P〈0．01），且随胃癌TNM分期的进展，其表达量逐渐增加。7种人胃癌细胞系中均有PAK4蛋白的表达，与GES-1细胞系相比，PAK4蛋自在ACTS、BGC823、MKN45和N87细胞系中呈高表达，而在MGC803、MKN1和SGC7901细胞系中呈低表达。结论PAK4蛋白的过度表达可能与人胃癌的发生发展及预后有关。Objective To investigate the expression of p21-activated kinase4 （PAK4） in human gastric cancer, and to evaluate the correlation of PAK4 protein level with clinical pathologic parameters. Methods Immunohistochemical method was employed for detecting the expression of PAK4 in tissue samples obtained from 95 gastric cancer patients who underwent surgical treatment. Western blot was also used to measure the expression of PAK4 in gastric cancer tissues and adjacent non-cancerous gastric mucosa in 40 patients and also in 7 human gastric cancer cell lines. Results Immunohistochemical staining results showed that positive PAK4 expression in the gastric cancer tissues was 67.4%, and 96.9% of those （62/64） was located in the cytoplasm, while only 3.1% （2/64） was on the cell membrane. Expression of PAK4 was revealed to be significantly and positively correlated with both TNM stage （Χ^2 = 10. 426, P 〈 0.01 ） and lymph node metastasis （Χ^2 =4. 912, P 〈0.05 ）. Western blot showed that PAK4 expression of gastric cancer tissues was significantly higher than that of adjacent non-cancerous gastric mucosa （ t = - 5. 978, P 〈 0.01 ）. Expression of PAK4 could also be detected in 7 human gastric cancer cell lines. Compared with GES-1 , expression of PAK4 is higher in MKN45, AGS, N87 and BGC823, but it was lower in MGCS03, MKN1 and SGC7901. Conclusion The overexpression of p21-activated kinase4 （PAK4） may be correlated with the carcinogenesis and prognosis of gastric cancer.

关 键 词：胃肿瘤 p21活化激酶4 胃癌组织 

分 类 号：R735.2[医药卫生—肿瘤]