纯钛镀制类金刚石薄膜的真菌黏附与细胞毒性研究  

Studies on Adhering of Candida Albicans and Cytotoxicity of Diamond Like Carbon Coating on Pure Titanium

作　　者：尹路 郭天文[1] 王辉[1] 赵雯[1] 弥谦[2] 惠迎雪[2] 

机构地区：[1]第四军医大学,陕西西安710032 [2]西安工业大学,陕西西安710032

出　　处：《稀有金属材料与工程》2008年第1期6-9,共4页Rare Metal Materials and Engineering

基　　金：国家自然科学基金(39770805);西安工业大学光电工程学院国家薄膜重点实验室开放课题基金资助(ZSKJ-200709)

摘　　要：采用脉冲电弧离子镀膜法在纯钛表面制备类金刚石膜(DLC)并在口内使用。利用表面粗糙度仪、分光光度仪、扫描电镜分别研究镀制类金刚石膜、氮化钛膜、阳极氧化膜以及未表面处理的齿科纯钛试件表面白色念珠菌黏附情况和细胞毒性。结果表明,类金刚石膜表面菌数最少,粗糙度增加值和反射率下降值最小。类金刚石膜表面的L-929细胞增值良好。类金刚石膜可以显著降低纯钛义齿表面的白色念珠菌黏附量,可以承受日常口腔刷牙的磨耗应用于人体,对齿科修复体具有大的潜在应用价值。Diamond-like carbon coatings were deposited onto pure titanium by Multi-arc pulse ion plating technique and used in vivo. Adhering of candida albicans of DLC, TiN, Anodizing and uncoated pure titanium was compared and the cytotoxicity of DLC was evaluated using scanning electron microscope, spectrophotometer, roughness apparatus and cell microscope. Experimental results indicate that the amount of candida albicans, change of roughness and reflectivity on DLC are least among the four groups of the samples. Surface morphology has little change. Cytotoxicity test shows DLC coatings are safe to L-929 cells. DLC coatings can endure daily brushing wear, are also safe to human, showing potential value in dental repair.

关 键 词：纯钛 类金刚石膜 真菌黏附 细胞毒性 

分 类 号：R318.08[医药卫生—生物医学工程]