过渡金属离子(Cu^(2+)、Cd^(2+))对藻红蛋白单体的漂白机制  

STUDY ON THE BLEACHING OF MONOMERS AND SUBUNITS OF RPHYCOERYTHRIN BY TRANSITION METALS(Cu2+, Cd2+)

作　　者：赵继全[1] 赵井泉[1] 张建平[1] 杨紫萱[1] 蒋丽金[1] 

机构地区：[1]中国科学院感光化学研究所

出　　处：《感光科学与光化学》1997年第2期145-150,共6页Photographic Science and Photochemistry

基　　金：国家自然科学基金

摘　　要：过渡金属离子（Ｃｕ２＋、Ｃｄ２＋）对藻红蛋白单体的漂白机制＊赵继全赵井泉张建平杨紫萱蒋丽金＊＊（中国科学院感光化学研究所，北京１００１０１）关键词漂白，藻红蛋白单体，藻红蛋白亚基，多肽链残基，色团络合物藻胆蛋白是一组同源捕光色素蛋白，由多肽链及相连的...he bleaching of the monomers and the separated α and β subnits of Rphycoerythrin from Polysiphonia urceolata by Cu2+, Cd2+, has been investigated. Upon the addition of Cu2+ and Cd2+, the visible absorption bands of the monomers at 496 , 538 and 564 nm of Rphycoerythrin was bleached. The change in absorption induced by Cu2+ or Cd2+ was accompanied by a decreased fluorescence emission intensity. The bleaching capacity of Cu2+ is greater than that of Cd2+. When monomers and α subunit are bleached by Cu2+, there is no appearance of the characteristic absorption of chromophore complex at 648 nm. But this absorption appeares obviously in the case of β subunit. From above results, we conclude the mechanism is that the bleachng of monomers and α subunit is caused by the interaction of Cu2+ or Cd2+ with the residues of polypeptides, but β subunit is bleached by both of the interaction of Cu2+ or Cd2+ with residues of polypeptides and the coordination of Cu2+ or Cd2+ with chromophores. 

关 键 词：漂白 藻红蛋白 光谱 多肽链 色团 铜离子 镉离子 

分 类 号：Q946.16[生物学—植物学]