结肠腺瘤性息肉蛋白、β-连接素和E-钙黏素在大肠癌中的表达  

Expression and significance of APC,β-catenin and E-cadherin proteins in colorectal carcinoma

作　　者：戴文斌[1] 任占平[1] 陈蔚麟[1] 杜娟[1] 石喆[1] 唐德艳[1] 

机构地区：[1]广西壮族自治区柳州市人民医院,柳州545001

出　　处：《陕西医学杂志》2008年第1期94-97,共4页Shaanxi Medical Journal

基　　金：广西壮族自治区柳州市科技攻关项目(2003自0217)

摘　　要：目的:探讨结肠腺瘤性息肉蛋白(APC)、β-连接素(β-catenin)和E-钙黏素(E-cadherin)在大肠癌发生、发展中的作用。方法:采用免疫组化方法检测30例正常大肠黏膜、30例大肠腺瘤、10例大肠腺瘤恶变及50例大肠癌组织中APC、β-catenin和E-cadherin蛋白的表达情况。结果:大肠癌和大肠腺瘤恶变APC阳性率分别为44.0%,40.0%,显著低于大肠腺瘤(86.7%)和正常大肠黏膜(100%)。大肠癌、大肠腺瘤恶变和大肠腺瘤β-catenin胞浆(胞核)异位表达率分别为62.0%,50.0%,30.0%,显著高于正常大肠黏膜(0%),大肠癌β-catenin异位表达率显著高于大肠腺瘤。大肠癌中β-catenin、E-cadherin膜表达缺失率为46.0%,34.0%,显著高于大肠腺瘤(10.0%,6.67%)和正常大肠黏膜(0%,0%),且与大肠癌组织分化程度、浸润深度、淋巴结转移、Dukes分期有关。APC蛋白表达与大肠癌组织分化程度有关。大肠癌中β-catenin异位表达与APC阳性表达呈负相关关系;β-catenin与E-cadherin胞膜阳性表达呈正相关关系。结论:APC失表达和(或)β-catenin异位表达与大肠癌的发生密切相关,可能是大肠癌发生的早期事件;β-catenin、E-cadherin膜表达缺失与大肠癌的侵袭、转移有关。Objective： To investigate the role of APC,β-catenin and E-cadherin in the carcinogenesis and progression of colorectal carcinoma. Methods： Expression of APC, β-catenin and E-cadherin proteins was examined immunohistochemically in 30 cases of normal colorectal mucosa, 30 cases of colorectal adenoma, 10 cases of colorectal adenoma carcinogenesis and 50 cases of colorectal carcinoma. Results： The positive expression rates of APC were 44. 0% and 40. 0% respectively in colorectal carcinoma and colorectal adenoma carcinogenesis, and both of the rates were significantly lower than that of colorectal adenoma （86. 7%） and normal colorecta] mucosa（100.0%）. The cytoplasmic and nuclear β-catenin expression rates were 62.0%, 50. 0% and 30.0% respectively in colorectal carcinoma, colorectal adenoma carcinogenesis and colorectal adenorna, and all of the rates were significantly higher than that of normal colorectal mucosa（0%）. The cytoplasmic and nuclear β-catenin expression rate in colorectal carcinoma was significantly higher than that of colorectal adenoma. The reduced membranous β-catenin and E-cadherin expression rates were 46.0% and 34.0% , and both of the rates were significantly higher than that of colorectal adenoma （10. 0%, 6. 67%） and normal colorectal mucosa （0%, 0%）. The reduced membranous β-catenin and E-cadherin expression were closely related with the tissue defferentiation degree, the depth of invasion, lymph node metastasis and Dukes stage in colorectal carcinoma. The cytoplasmic and nuclear ]3 catenin expression was in negative correlation with the expression of APC. The membranous βcatenin expression was thus in positive correlation with the expression of E-cadherin. Conclusions： The reduced cytoplasmic APC expression , the cytoplasmic and nuclear β-catenin expression may play a pivotal role in carcinogenesis and progression of colorectal carcinoma, and may be an early event . The reduced membranous β-catenin and E-cadherin expression may be related to the invasion and me

关 键 词：肠肿瘤/病理学 腺瘤状结肠息肉蛋白/代谢 连接蛋白类/代谢 

分 类 号：R734.2[医药卫生—肿瘤] R735.34[医药卫生—临床医学]