人脐静脉内皮细胞体外流动培养系统的制作研究  

Study on fabrication of flow channel for cells culture in vitro

作　　者：张莉[1] 蔡绍皙[1] 刘冰[1] 蒋稼欢[1] 韩亚刚[1] 

机构地区：[1]重庆大学生物工程学院

出　　处：《重庆医学》2008年第2期113-114,118,共3页Chongqing medicine

基　　金：国家自然科学基金资助项目(10272122);国家基金委周际合作局资助项目(10411120690)

摘　　要：目的构建一个能用于体外研究细胞力学行为的流动腔系统,它能在体外模拟动脉粥样硬化好发部位复杂的血液流动状态。方法采用软刻法制作聚二甲基硅氧烷(PDMS)流动腔,对PDMS流动腔的加工工艺、封装方法和腔内细胞生长进行了探讨。结果获得了光学性能良好并能模拟体内特殊流型的流动腔,可承受较大范围的剪切力,细胞能在腔底正常生长。结论该加工方法快速、简便,制作的PDMS流动腔可进一步用于体外细胞力学行为的实时研究。Objective To simulate the blood flow features of the susceptible sites of atherosclerosis for studying cell＇s mechanical behavior in vitro. Methods Polydimethyle syloxane（PDMS） flow channel was fabricated by soft lithography. The methods of fabrication and enclose of PDMS channel and cell culture on the bottom of channel were studied in detail. Results The PDMS chamber which possessed favorable optical property was obtained. It could endure wide-range shearing force. Cells grew normally on fibronectin bottom of channel. Conclusion The fabricated methods are quick and simple. The prepared channel could be used for fhe advanced real-time investigations of cells mechanical behavior in vitro.

关 键 词：聚二甲基硅氧烷(PDMS) 流动腔 微机电系统 软刻法 

分 类 号：R318.6[医药卫生—生物医学工程]