采用四硫富瓦烯为黄嘌呤氧化酶的电子传递体的次黄嘌呤传感器  

Hypoxanthine Sensor with Tetrathiafulvalene as Electron Shuttle between Xanthine Oxidase and Glassy Carbon Electrode

作　　者：张晓林[1] 吴芯芯[1] 邓家祺 漆德瑶[1] 

机构地区：[1]上海大学化学化工系

出　　处：《分析测试学报》1997年第1期17-21,共5页Journal of Instrumental Analysis

基　　金：国家自然科学基金;中国科学院长春应用化学研究所电分析化学开放实验室资助

摘　　要：采用四硫富瓦烯（ＴＴＦ）作为黄嘌呤氧化酶与玻碳电极之间的电子传递体，通过牛血清白蛋白和戊二醛交联剂，把黄嘌呤氧化酶固定在Ｎａｆｉｏｎ－ＴＴＦ修饰玻碳电极上，制备成次黄嘌呤传感器。该传感器的线性范围为１．０×１０－５～７．５×１０－４ｍｏｌ／Ｌ，响应时间小于６０ｓ。Tetrathiafulvalene (TTF) is employed to expedite electron exchange between xanthine oxidase and glassy carbon electrode.Amperometric sensor for hypoxanthine has been deve loped by immobilizing glucose oxidase on Nafion TTF modified carbon electrode with bovine serum albumin cross linked by glutaraldehyde.The role of Nafion membrane is to prevent the loss of tetrathiafulvalene and the oxidation of unwanted electrochemically active species on the electrode.The biosensor responds rapidly to hypoxanthine within 60 s and its linear range is 1.0×10 5 ～7.0×10 4 mol/L.

关 键 词：生物传感器 NAFION 次黄嘌呤 黄嘌呤氧化酶 TTF 

分 类 号：Q－331[生物学]