结直肠癌组织中Tiam1,Fascin-1及HSPB1的表达及意义  

Expression and significance of Tiam1,Fascin-1 and HSPB1 in human colorectal cancer

作　　者：丁轶[1] 刘莉[2] 蒋会勇[2] 丁彦青[2] 

机构地区：[1]南方医科大学南方医院放疗科,广州510515 [2]南方医科大学南方医院病理科,广州510515

出　　处：《广东医学》2008年第2期230-232,共3页Guangdong Medical Journal

基　　金：国家自然科学基金资助项目(编号:30500242)

摘　　要：目的探讨结直肠癌组织中Tiam1,Fascin-1及HSPB1的表达及意义。方法制作含100例结直肠癌组织的组织芯片,应用免疫组化SP法检测结直肠癌组织中Tiam1,Fascin-1及HSPB1的表达。结果组织芯片免疫组化染色后,形态可观测率为96%,并且背景清晰,对比鲜明。Tiam1表达阳性率为74%,Fascin-1表达阳性率为51%,HSPB1表达阳性率为68%,显著高于非肿瘤组织。Tiam1,Fascin-1及HSPB1表达与结直肠癌转移显著相关,伴发转移的结直肠癌组织Tiam1,Fascin-1及HSPB1阳性表达明显高于无转移者。通过相关性统计学分析,发现Tiam1与Fascin-1表达呈正相关(r=0.678,P<0.01),Tiam1与HSPB1表达呈正相关(r=0.650,P<0.01)。结论Tiam1,Fascin-1及HSPB1均与结直肠癌转移有关,Fascin-1和HSPB1的高表达可能与Tiam1的调控有关。Objective To investigate the expression of T lymphoma invasion and metastasis gene 1 （ Tiam1 ）, Fascin - 1 and heat shock 27kDa protein 1 （HSPB1） in human colorectal cancer （CRC） and their significance. Methods A tissue microarray （TMA） containing 100 informative cases of CRC was constructed. Immunohistochemistry SP method was used to detect the expression of Tiam1, Fascin - 1 and HSPB1 in TMA of CRC respectively. Results Ninety -six percents of dots on the TMA were observable morphologically with clear background and vivid contrast after immunohisto- chemistry. The positive rates of Tiam1, Fascin - 1 and HSPB1 were 74%, 51% and 68% respectively in TMA of colorectal cancer. Expression of Tiam1, Fascin - 1 and HSPB1 protein in CRC with metastasis was higher than those in CRC without metastasis. This finding indicated that Tiam1, Fascin - 1 and HSPB1 were positively correlated with metastasis of CRC. We next analyzed the correlation among Tiam1, Fascin - 1 and HSPB1. Tiam1 expression was positively correlated with Fascin - 1 （r=0.678, P〈0.01） and HSPB1 （r=0.650, P〈0.01） in colorectal cancer. Conclusion Tiam1, Fascin-1 and HSPB1 are related to metastasis of CRC. Overexpression of Fascin - 1 and HSPB1 may relate to Tiam1 regulation.

关 键 词：TIAM1 FASCIN-1 HSPB1 结直肠肿瘤 转移 

分 类 号：R735.2[医药卫生—肿瘤]