安吉白茶正常与白化叶片基因表达差异的初步研究  

Preliminary Study on Gene Expression Differences between Normal Leaves and Albino Leaves of Anji Baicha (Camellia sinensis cv. Baiye1)

作　　者：王新超[1] 赵丽萍[1] 姚明哲[1] 陈亮[1] 杨亚军[1] 

机构地区：[1]中国农业科学院茶叶研究所茶树资源和遗传改良与分子生物学实验室侬业部茶叶化学工程重点实验室,浙江杭州310008

出　　处：《茶叶科学》2008年第1期50-55,共6页Journal of Tea Science

基　　金：浙江省自然科学基金(Y304448);农业部茶叶化学工程重点实验室开放基金(200403)内容

摘　　要：用mRNA差别显示技术(DDRT-PCR)研究了茶树温敏突变体——安吉白茶正常叶片和白化叶片的基因表达差异。从58个差异表达的片段中通过半定量RT-PCR鉴定出12个阳性片段,其中5个片段在正常叶片中特异表达,4个在白化叶片中特异表达,1个在绿色叶片中上调表达,2个在白化叶片中上调表达。通过与GenBank BLASTX比对分析,5个基因片段比对出相似序列,分别为拟南芥的血红素结合蛋白家族中心区域基因、甜菜的甲硫氨酸合酶基因、苜蓿的一个逆转座子基因、人类20号染色体上的一段3-磷酸甘油醛脱氢酶假基因和玫瑰的ACC合成酶基因,其余7个片段未比对上同源序列,可能为新基因。Differences of gene expression between normal and albino leaves of tea plant （Camellia sinensis cv. Baiyel） were studied by mRNA differential display reverse transcription-PCR （DDRT-PCR）. Fifty-eight differentially expressed cDNA fragments were obtained from normal and albino leaves. Twelve of which were confirmed by the analysis of RT-PCR. Among them, 5 fragments were specially expressed in normal leaves, 4 fragments were specially expressed in albino leaves, 1 fragment was up-regulated in normal leaves, and 2 fragments were up-regulated in albino leaves. Through BLASTX analysis with GenBank, 5 cDNA fragments showed high homologous to five known genes, namely soul heme-binding family protein of Arabidopsis thaliana, methionine synthase of Beta vulgaris, gag-pol polyprotein-related in Medicago truncatula, human glyceraldehyde-3-phosphate dehydrogenase pseudogene and ACC synthase of rose, respectively. The other 7 might be novel genes, they showed poor homologous to all proteins in the database.

关 键 词：安吉白茶 白化 基因表达差异 MRNA差别显示 RT—PCR 

分 类 号：S571.1[农业科学—茶叶生产加工] Q523[农业科学—作物学]