6种舌鳎亚科鱼类ITS1序列长度多态性及系统分析  被引量:29

PHYLOGENETIC RELATIONSHIP AND LENGTH VARIATION IN THE FIRST RIBOSOMAL INTERNAL TRANSCRIBED SPACER OF CYNOGLOSSINAE SPECIES

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作  者:徐晖[1] 李军[2] 孔晓瑜[3] 肖志忠[2] 李玉龙[1] 张艳春[1] 时伟[1] 位正鹏[1] 喻子牛[3] 

机构地区:[1]中国海洋大学海水养殖教育部重点实验室,青岛266003 [2]中国科学院海洋研究所,青岛266071 [3]中国科学院南海海洋研究所,广州510301

出  处:《海洋与湖沼》2008年第1期35-41,共7页Oceanologia Et Limnologia Sinica

基  金:山东省科技计划项目,2005GG3205071号;中国海洋大学海水养殖教育部重点实验室开放课题资助,200606号

摘  要:利用核糖体第一内转录间隔区(ITS1)对舌鳎亚科(Cynoglossinae)6种鱼类进行系统分析,发现舌鳎亚科ITS1区具有明显的序列长度多态性(404—744bp),序列长度分为三种类型,分别为404—405bp、462—463bp以及741—744bp,同一长度类型的不同种类间序列高度相似,平均遗传距离分别为0.00248、0.00217和0.00169,而不同类型间序列差异显著,平均遗传距离最小为0.38453。分析表明,序列长度多态性可能与物种分化时间有关。采用NJ(neighbour-joining)法及MP(maximum parsimony)法构建分子系统树,结合形态学特征及GenBank中的线粒体DNA序列进行分析,表明紫斑舌鳎(Cynoglossus purpureomaculatus)与短吻三线舌鳎(C.abbreviatus)可能为同物异名。另外,舌鳎属(Cynoglossus)的中华舌鳎(C.sinicus)与须鳎属(Paraplagusia)的日本须鳎(Paraplagusia japonica)聚为一支,舌鳎属中三线舌鳎亚属(Areliscus)的长吻红舌鳎(C.lighti)与拟舌鳎亚属(Cynoglossoides)的少鳞舌鳎(C.oligolepis)聚为一支,与形态分类学的观点不一致,值得进一步研究。Previous studies on phylogenetic relationship of Cynoglossinae fish species relied mostly on morphological data. However, the morphology-based phylogeny and classification of them remain controversial. Recently, combined with morphological data, large advancement has been made on molecular level and has yielded interesting results in regard to tonguefish taxa. In this study, the first ribosomal internal transcribed spacers (ITS1) of nuclear ribosomal DNA from six species of the subfamily Cynoglossinae were used for phylogenetic analysis. The significant length variation of ITS1 region was observed, with range of 404-744bp in length. Clade were established of three well-morphologically-defined groups. The first group, including C. purpureomaculatus and C. abbreviatus, was 404-405hp long; C. sinicus and P. japonica was, as the second group, in length of 462-463bp; and C. oligolepis and C. lighti, the last group, was 741-744bp long. The alignment length of ITS 1 was 756 long, with variable sites of 286, of which 285 were parsimony informative. High variability was detected in the ITS 1 region, and the conserved motifs were very short except for the motif (ACCCAACTCTCC), which was common for all species. The sequences contained relatively high GC content (from 66.2% to 69.5%), averaging 67.2%. The mean intra-group distance was 0.00248, 0.00217 and 0.00169 respectively, and the mean in-ter-group distance between Groups Ⅰ and Ⅱ was 0.3845, between Ⅱ and Ⅲ was 0.9088, and between Ⅰ and Ⅲ was 0.8685. The intra-group distances were very smaller than those of inter-group, suggesting that the species in the same group have very close relationship in evolution. The DNA data of these fishes were processed using neighbor-joining (NJ) and maximum parsimony (MP) methods to build phylogenetic relationships within the subfamily of them. Based on morphological, nuclear DNA and mitochondrial DNA data available so far, it is regarded that C. abbreviatus and C. purpureomaculatus could be the same speci

关 键 词:舌鳎亚科 ITS1 长度多态性 系统发生关系 

分 类 号:Q953[生物学—动物学]

 

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