VEGF诱导的大鼠视网膜白细胞聚集与血-视网膜通透性改变的相关性研究  

VEGF induced retinal leukocyte accumulation and blood retinal barrier breakdown in rats

作　　者：王康[1] 李明铭[1] 李新民[1] 王艳玲[1] 

机构地区：[1]首都医科大学附属北京友谊医院眼科,北京市100050

出　　处：《眼科新进展》2008年第1期16-17,20,共3页Recent Advances in Ophthalmology

基　　金：北京市科委科技新星项目基金资助(编号:2005-B-043);北京市优秀人才培养专项经费基金资助(编号:20042D0300102)~~

摘　　要：目的探讨血管内皮生长因子(vascular endothelial growth factor,VEGF)诱导的大鼠视网膜白细胞聚集及其与血-视网膜通透性改变的关系。方法12只Brown-Norway大鼠分为对照组及VEGF注射组,吖啶橙(5mg·kg-1)和伊凡思蓝(45mg·kg-1)经颈静脉注入,分别检测视网膜中白细胞密度和伊凡思蓝含量。结果VEGF注射24h后,对照组和VEGF注射组大鼠视网膜白细胞密度分别为(1.90±0.03)×10-6μm-2和(9.10±0.08)×10-6μm-2;视网膜标准化伊凡思蓝含量分别为(14.78±3.27)ng·mg-1和(51.72±6.77)ng·mg-1,VEGF注射组与对照组比较差异均有显著统计学意义(P<0.01)。结论VEGF注射后视网膜白细胞聚集和血管通透性显著增加,可以通过吖啶橙造影和伊凡思蓝定量检测。Objective To investigate the relationship between retinal leukocyte accumulation induced by vascular endothelial growth factor（VEGF） and blood retinal barrier breakdown in rats.Methods Twelve Brown-Norway rats were divided into control group and VEGF intravitreal injection group.After acridine orange （5 mg·kg^-1） and evans blue（45 mg·kg^-1） injecting through the jugular vein,the density of leukocytes and content of evans blue were detected in the retina.Results Twenty-four hours after VEGF intravitreal injection,the density of leukocytes in the retina of control group and VEGF intravitreal injection group were （1.90±0.03）×10^-6 per square micrometer and （9.10±0.08）×10^-6 per square micrometer,and the content of evans blue were （14.78±3.27）ng·mg^-1 and（51.72±6.77）ng·mg^-1.VEGF intravitreal injection groups were much higher than that of control groups（P〈0.01）.Conclusion Retinal leukocyte accumulation induced by VEGF has relations with the increase of blood retinal barrier breakdown,which can be quantitatively detected by the methods of acridine orange fluorography and evans blue.

关 键 词：吖啶橙造影 伊凡思蓝 大鼠 血-视网膜屏障 血管内皮生长因子 

分 类 号：Q959.836[生物学—动物学] R774[医药卫生—眼科]