bFGF在体外培养的正常人及白内障患者LECs中的表达  

Expression of bFGF in lens epithelial cells of normal people and cataract patients in vitro

作　　者：夏朝霞[1] 吴艺[2] 蓝育青[1] 穆剑[1] 肖剑晖[1] 

机构地区：[1]中山大学附属第二医院眼科,广东省广州市510120 [2]广东省第二人民医院眼科,广东省广州市510317

出　　处：《眼科新进展》2008年第1期21-25,共5页Recent Advances in Ophthalmology

基　　金：广东省重点科技项目基金资助(编号:2KM05204S)~~

摘　　要：目的体外培养正常人及白内障患者晶状体上皮细胞(lens epithelial cells,LECs),分别检测碱性成纤维细胞生长因子(basic fibroblast growth factor,bFGF)在体外培养的正常人及白内障患者LECs中的表达,以探讨bFGF在白内障患者LECs中的作用。方法体外培养正常人及白内障患者LECs并传代;利用免疫组织化学法和免疫印迹法,检测bFGF在体外培养的正常人及白内障患者LECs不同阶段的蛋白水平的表达。结果原代培养的正常人及白内障患者LECs无明显差异,但在传代过程中白内障患者的LECs出现了较早的细胞衰老现象;在正常人原代培养细胞和传代的LECs中检测不到bFGF的表达,或仅有微弱表达,而在白内障患者的原代培养细胞和传代的LECs中,bFGF的表达较正常人明显增多,并明确表达了相对分子质量为17000的蛋白。结论老年性白内障LECs相对正常LECs而言,生长缓慢,容易出现细胞衰老现象,且有丝分裂能力差;白内障患者术后残留的LECs引起损伤修复过程,合成并释放bFGF,造成LECs的增生、移行和纤维化,并可能成为后囊混浊的原因之一。Objective To detect the expression of basic fibroblast growth factor（bFGF） in lens epithelial cells （LECs） of normal person and cataract patients cultured in vitro respectively.Methods LECs of normal and cataract persons were cultured in vitro and passaged.The expression of bFGF protein in LECs of normal person and cataract patients cultured in vitro at different passages were detectd by immunohistochemical staining and Western blots methods.Results There was no obvious morphological difference between the cells of primary passage from cataract patients and those from the normal person.Only two times later of passage,the LECs of cataract group appeared the change of aging compared with normal group.No expression or only faint expression of bFGF was found in the primary passage or in subcultured passage.The expression of bFGF increased obviously in LECs of cataract patients than in normal group.The results showed that the bFGF protein of Mr 17 000 could be identified in LECs of cataract patient.Conclusion LECs of normal people have better proliferation ability than those of senile cataract.There was no expression or only faint expression of bFGF in the primary passage or in subcultured passage of normal person cultured in vitro.The expression of bFGF increase obviously in LECs of cataract patients.The remnants LECs of postoperation can arouse damnification and rehabilitation courses,in which bFGF was synthesized and released.Thus the proliferation,metaplasia,and myofibroblast of LECs are induced,which may be one reason of posterior capsule opacification.

关 键 词：晶状体上皮细胞 碱性成纤维细胞生长因子 免疫组织化学 Western BLOT 

分 类 号：R776.1[医药卫生—眼科]