颞叶癫大鼠海马内Rnd1 mRNA及蛋白表达变化  

Expression Alteration of Rnd1 mRNA and Protein in Rat Hippocampus of Temporal Lobe Epilepsy

作　　者：柳茵[1] 肖波[1] 周艳辉[1] 冯莉[1] 龙莉莉[1] 李国良[1] 龙小艳[1] 

机构地区：[1]中南大学湘雅医院神经内科,410008

出　　处：《中国临床神经科学》2008年第1期11-17,共7页Chinese Journal of Clinical Neurosciences

基　　金：教育部高等学校优秀青年教师教学科研奖励计划(2001-182)

摘　　要：目的:动态观察小分子GTPase Rho家族的Rnd1 mRNA及其蛋白在氯化锂-毛果芸香碱(匹罗卡品)致大鼠模型海马中的表达变化,探讨其在颞叶癫发生发展中的作用。方法:在氯化锂-毛果芸香碱颞叶癫模型中应用逆转录聚合酶链反应(RT-PCR)检测癫持续发作(SE)后各时间点海马内Rnd1 mRNA的表达变化,并运用免疫组织化学染色法及Neo-Timm染色法分别检测齿状回门区、CA1区及CA3区中该蛋白在不同时间点的表达变化及苔藓纤维出芽(MFS)情况。结果:实验发现模型组于SE后8h内即出现Rnd1表达上调,SE后约1d达高峰,7d左右回复至对照组水平,此后其mRNA表达水平与对照组相似;而免疫组化染色发现Rnd1蛋白表达从SE后8h内即开始上调,约3d达高峰,至7d虽略有回落,但仍高于对照组水平,且这种情况可一直持续至慢性期。结论:急性期海马齿状回门区Rnd1表达上调可能通过促进MFS的发生参与了颞叶癫的发生。Aim： Our aim is to investigate mRNA and protein expression alterations of Rndl in hippocampi of LiCl-pilocarpine epileptic rats, as to further explore the mechanisms of mossy fiber sprouting and epileptogenesis. Methods： LiCl and pilocarpine were given intraperitoneally to establish temporal lobe epilepsy rat model. Expression alterations of Rndl mRNA and protein in hippocampus were examined by RT-PC R and immunohistochemical staining respectively at different time point, while mossy fiber sprouting was examined by Neo-Timm staining. Results： Rnd1 mRNA upregulation occurred within 8h post status and maximized around ld post status, which returned to normal level until 7 d post status; while protein upregulation started as early as 8 h post status, and maximized around 3 d post status. Though protein level turned down since 7 d post expression, it remained above control level and the upregulation persisted in chronic stage. Conclusion： Hippocampal Rndl upregulation in acute stage may participate in the initiation of mossy fiber sprouting in temporal lobe epilepsy.

关 键 词：颞叶癫痫 毛果芸香碱 Rnd1蛋白 苔藓纤维出芽 

分 类 号：R742.1[医药卫生—神经病学与精神病学]