化疗药物不同剂量对胶质瘤细胞U251化疗敏感性的影响  

Effect of Different Doses of Chemotherapeutic Agents on Sensitivity of Glioma U251 Cell Line to Chemotherapy.

作　　者：金许洪[1] 马跃辉[2] 王明[2] 徐庆生[2] 周永庆[2] 邬东晓[1] 何国龙[1] 秦福创[1] 

机构地区：[1]杭州市红十字会医院神经外科,310003 [2]浙江大学医学院附属第一医院神经外科

出　　处：《医学研究杂志》2008年第1期20-22,129,共4页Journal of Medical Research

基　　金：浙江省卫生厅基金资助(编号:2003A028);杭州市科技局资助项目(编号:20061133B12)

摘　　要：目的检测不同剂量化疗药物对胶质瘤细胞U251化疗敏感性的影响。方法利用MTT法检测不同化疗药物(阿霉素、长春新碱、足叶乙甙以及联合异搏定)在不同剂量的作用下,胶质瘤细胞U251的存活度。结果单用阿霉素、长春新碱、足叶乙甙,细胞存活度均随着化疗药物浓度增加而减少,并且在一定浓度范围内细胞存活度随着化疗浓度增加而减少的幅度较大;浓度较低或较高时,细胞存活度变化的趋势不大。联合异搏定对部分化疗药有一定的协同作用。结论化疗药物的剂量与化疗敏感性存在一定关系,为临床化疗选择合适的化疗剂量提供了实验依据。Objective To study the effect of chemotherapeutic agents of different doses on sensitivity of glioma U251 cell line to chemotherapy. Methods The rates of cell growth of the U251 cell line under the effect of different chemotherapeutic agents （DOX,VCR, VP - 16 and with verapamil） of a series of doses were detected by MTT assay. Results Using DOX or VCR or VP - 16 singly, the rates of cell growth were detected at a lever with positively correlates with the concentrations of these chemotherapeutic agents. Moreover the rates of cell growth changed more obviously within some range of concentrations, while the change is limited with the high or low concentrations. The combination of pates of chemotherapeutic agents and verapamil show synergistic effect. Conclusions The relationship between the doses of chemotherapeutic agents and sensitivity of chemotherapy gas a basis for choosing appropriate clinical chemotherapy doses.

关 键 词：化疗剂量 胶质瘤 化疗敏感性 

分 类 号：R730.53[医药卫生—肿瘤] R730.264[医药卫生—临床医学]