TCT联合HPV-DNA检测在宫颈病变中的诊断价值  

Diagnostic Value of TCT join to HPV-DNA Testing in Cervical Disease.

作　　者：廖丹梅[1] 黄明春[1] 李惠珍[1] 甘柏柳[1] 覃军[1] 王燕娇[1] 陆和屏[1] 叶朝阳[1] 

机构地区：[1]广西南宁市妇幼保健院,530011

出　　处：《医学研究杂志》2008年第1期81-84,共4页Journal of Medical Research

基　　金：广西南宁市科学研究与技术开发计划项目(200501086C)

摘　　要：目的宫颈癌是妇女常见的恶性肿瘤之一。目前大量的研究认为人乳头瘤病毒(HPV)是宫颈癌的主要致病因素,几乎所有宫颈癌都伴有HPV亚型。本研究旨在评价TCT联合HPV-DNA检测对宫颈病变的诊断价值。方法用新柏液基薄层细胞学(TCT)技术筛查并与病理学诊断相结合,对宫颈炎和宫颈上皮内瘤变(CIN1)一级及其以上(≥CIN1)病例各200例进行高危型HPV16/18型荧光定量PCR检测。结果宫颈病变≥CIN1组阳性病例69例,阳性率34.5%;宫颈炎对照组阳性病例16例,阳性率8.0%。两组比较差异有统计学意义(P<0.01)。结论高危型HPV16/18感染与宫颈上皮内瘤变密切相关。HPV高危型患者CIN发病率较高,特别是高度病变,HPV高危型检测在宫颈病变的检查中有重要意义。如与宫颈细胞学检查(TCT技术)、阴道镜检查和病理诊断相结合可提高宫颈病变的检出率。Objective Cervical cancer is a usual malignency of women, a large number of stadies consider that human papillomavirus（HPV） is the primary risk factor of cervical cancer and almost all of the cervical cancer occur with sub - type HPV. the Objective of this study is to evaluate the diagnostic value of TCT join to HPV - DNA test. Methods Uing ThinPrep cytology test（TCT） join to pathologic diagnosis and high risk HPV16/18 fluorescent quantitative analysis PCR respectively tested 200 cases witch diagnosed CIN1 or 〉 CIN1. Results There are 69 positive cases in ≤ C IN1 group, positive rate is 34.5% ; There are 16 positive cases in cervicitis group, positive rate is 8.0%. these two groups have statistic difference. Conclusions High risk type HPV16/18 infection correlate with CIN. Patients with high risk type HPV have higher risk of C IN, the High risk type HPV16/18 test is important in cervical disease. If join to TCT , colposcope and pathologic diagnosis, detectable rate will improve.

关 键 词：TCT检测 宫颈上皮内瘤病变 HPV高危型检测 病理学诊断 

分 类 号：R737.33[医药卫生—肿瘤] R711.74[医药卫生—临床医学]