大鼠第一腮弓细胞构建组织工程牙本质-牙髓复合体样结构  

Tissue engineering of dentin-pulp complex-like structures by rat first branchial arch cells

作　　者：邓蔓菁[1] 陈小红[1] 刘鲁川[1] 安建平[1] 匡桂英[1] 

机构地区：[1]第三军医大学大坪医院野战外科研究所口腔科,重庆400042

出　　处：《第三军医大学学报》2008年第1期57-59,共3页Journal of Third Military Medical University

基　　金：第三军医大学科研创新基金(2004);重庆市自然科学基金(2005BB5307;2007BB5075)~~

摘　　要：目的观察胚胎12.5d(E12.5d)大鼠第一腮弓上皮及外胚间充质细胞离散后肾被膜下移植的成牙能力。方法切取E12.5d大鼠第一腮弓(下颌突),酶消化离心后与明胶海绵支架复合植入大鼠肾被膜下,4周后收获移植物,对移植物进行组织学观察和免疫组化抗牙本质涎蛋白(dentin sialoprotein,DSP)检测。结果种植4周,植入细胞在肾被膜下形成牙本质-牙髓复合体样结构。DSP在新生的牙本质组织中呈阳性表达。Masson三色法显示绿色矿化基质形成。结论E12.5d大鼠第一腮弓细胞部分保留了牙齿自然发育的遗传信号,在肾被膜下可以构建形成牙本质-牙髓复合体样结构。Objective To evaluate the odontogenetic ability of first branchial arch cells of El2.5 rats. Methods First branchial arch cells （mandibular process） of El2.5 SD rats were isolated enzymatically and collected. After combined with gelatin sponge, the cells were transplanted into the renal capsule of a rat. The specimen was taken out and evaluated by histological and immunohistochemical methods in 4 weeks after growth in renal capsule. Results The first branchial arch cells with gelatin sponge developed dentin-pulp complexlike structure. Dentin sialoprotein （DSP） was expressed in the newly formed dentin-like structure. The green mineralized matrix was further identified with Masson＇ s trichrome staining. Conclusion Cells from first bran- chial arch of El2.5 rats can partially keep genetic signal of tooth growth and form dentin-pulp complex-like structure in renal capsule.

关 键 词：第一腮弓细胞 牙本质-牙髓复合体 牙齿发育 

分 类 号：R318.08[医药卫生—生物医学工程] R782[医药卫生—基础医学]