胃癌细胞株SGC-7901生长抑素2型受体表达和外源性生长抑素类似物的影响  

The Inhibitory Effects of Somatostatin Analogue on the Expression of SomatostatinReceptor 2 in Gastric Cancer SGC- 7901 Cells

作　　者：王承党[1] 刘霞[1] 陈滟珊[1] 

机构地区：[1]福建医科大学附属第一医院消化内科,福建医科大学消化系病研究室,福州市350005

出　　处：《中国肿瘤临床》2008年第2期109-112,共4页Chinese Journal of Clinical Oncology

基　　金：国家教育部高等院校骨干教师基金(编号:00A003);福建省自然科学基金资助(编号:C9910008)

摘　　要：目的:观察胃癌细胞株SGC-7901生长抑素2型受体(SSTR-2)表达及外源性生长抑素类似物(SSA)对胃癌细胞生长和SSTR-2 mRNA表达的影响。方法:体外培养人胃癌细胞株SCG-7901,制作细胞爬片,免疫细胞化学染色法检测其SSTR-2表达。在RPMI-1640培养液中加入SSA,使SSA终浓度分别为0、10~4、10~5、10~6mmol/L,培养0、24、36、48、72h,MTT法测定肿瘤细胞生长情况、RT-PCR法和计算机图像分析法,检测SSTR-2 mRNA表达情况。结果:免疫细胞化学染色法检测显示100%的SGC-7901细胞呈SSTR-2染色阳性,主要位于细胞膜上,部分胞浆也有染色。培养24h～72h,1x10^4～1×10~6mmol/L浓度的SSA可以使肿瘤细胞生长平均被抑制17.86%～40.48%。当SSA浓度为1×10~4和1×10~5mmol/L时,72h时其抑制率分别为17.86%和21.43%,与其他时间点的差异有显著性意义(P<0.001和P<0.01)。当SSA浓度为1×10~6mmol/L时,对肿瘤细胞的抑制作用呈时间依赖性,72h时抑制率达到40.48%,显著高于1×10~4和1×10~5mmol/L组(P<0.001)。SGC7901细胞SSTR-2mRNA表达阳性;当SSA浓度为1×10~4、1×10~5mmol/L时,SSTR-2mRNA表达下调1.35%～31.65%(P<0.05),其中36h、48h、72h时低于0和24h时(P<0.01)。SSA浓度为1×10~6mmol/L时,各时间点的OD值差异没有统计学意义(P>0.05)。结论:SGC-7901胃癌细胞株高表达SSTR-2 mRNA和蛋白。适当浓度的SSA对SGC-7901细胞的抑制作用才呈现时间依赖性,并对SSTR-2mRNA表达没有影响。Objective： To investigate the expression of somatostatin receptor 2 （SSTR-2） in gastric cancer SGC- 7901 cells and to observe the effects of somatostatin analogues （SSA） on （cell proliferation and the expression of this receptor. Methods： SGC-7901 cells were cuhured in vitro. The expression of SSTR-2 was assessed by SP immunoeyto- chemistry. After these cells were treated with SSA at 0, 1×10^-4, 1×10 ^-5, and 1×10^-6mmol/l for 24h. 36h, 48h, and 72h, the cell proliferation was detected by MTF assay and the expression of SSTR-2 mRNA was assessed by RT-PCR. Results： The positive rate of SSTR-2 in SGC-7901 cells was 100%. SSTR-2 was located mainly on the membrane and partially in the cytoplasm. Those cells treated with SSA （1×10^-4-1×10^-6 mmol/l） for 24h-72h presented with a decrease （17.86%- 40.48%） in cell growth. SSA at 1 ×10^-6mmol/l had a time-dependent inhibito,,y effect on the cancer cell gTowth, and the maximal inhibitory tale was 40.48% at 72h. When SSA was administered at 1×10^-4 and I ×10^-5mmol/l, the expression of SSTR-2 mRNA was reduced hy 1.35%-31.65%. SSA at 1 ×10^-6 mmol/l did not affect the expression of SSTR-2 mRNA. Conclusion： The SSTR-2 was highly expressed on SGC-7901 cells. Without any effeel on the expression of SSTR-2 mRNA, SSA at appropriate concentrations can time-dependently inhibit the cell growth.

关 键 词：胃癌 生长抑素受体 生长抑素类似物 

分 类 号：R735.2[医药卫生—肿瘤] R735.9[医药卫生—临床医学]