新基因NOR1对肝癌细胞系HepG2蛋白质表达谱的影响  

Effects of a novel gene NOR_1 on the protein expression of HepG2 cell line

作　　者：聂新民[1] 周建大[2] 黄竹英[1] 桂嵘[1] 李登清[3] 唐华[3] 

机构地区：[1]中南大学湘雅三医院检验科,湖南长沙410013 [2]中南大学湘雅三医院烧伤整形科,湖南长沙410013 [3]中南大学湘雅医学院医学检验系,湖南长沙410013

出　　处：《细胞与分子免疫学杂志》2008年第2期164-166,共3页Chinese Journal of Cellular and Molecular Immunology

基　　金：国家自然科学基金资助项目(30300383);湖南省自然科学基金资助项目(07JJ3036)

摘　　要：目的:研究NOR1基因对肝癌细胞系HepG2蛋白质表达谱的影响。方法:将pcDNA3.1(+)/NOR1的表达质粒经脂质体导入HepG2细胞,Northern blot筛选出阳性克隆,通过双向电泳分离过表达NOR1的HepG2细胞内蛋白质,筛选出差异表达的蛋白质点,并进行质谱分析鉴定。结果:鉴定出6种表达上调的蛋白质点,包括锌指蛋白、肿瘤坏死因子受体、酪氨酸蛋白磷酸化受体等,这些蛋白质涉及基因转录、信号转导等肿瘤发生相关事件。结论:NOR1基因可能通过上调这些蛋白参与多种途径影响肝癌的发生发展。AIM： To investigate the mechanism of NOR1 gene, a novel gene associated with liver cancer. METHODS： NOR1 was introduced into HepG2 cells by liposome transfection. After staining and image analysis, 6 differential expression spots which were up-regulated in NOR1 transfected cells were isolated and identified by two-dimensional polyacrylamide gel eletrophoresis （2D PAGE ） and MALDI-TOF. RESULTS. The proteins which included zinc finger protein, tumor necrosis factor receptor, and protein tyrosine phosphatase receptor were involved in gene transcription and signal transduction associated with cancer. CONCLUSION： NOR1 gene may have some effects on liver cancer by up-regulating the expression of these proteins.

关 键 词：NOR1基因 双向电泳 基质辅助激光解吸飞行时间质谱 

分 类 号：R735[医药卫生—肿瘤]