地塞米松对体外大鼠晶状体上皮细胞增殖与分化的影响  

Effect of dexamethasone on proliferation and differentiation of rat lens epithelial cells in vitro

作　　者：陈华新[1] 杨磊[1] 张端莲[2] 

机构地区：[1]广州军区武汉总医院眼科,中国湖北省武汉市430070 [2]武汉大学医学院人体解剖和组织胚胎学教研室,中国湖北省武汉市430071

出　　处：《国际眼科杂志》2008年第1期32-35,共4页International Eye Science

摘　　要：目的:探讨地塞米松对大鼠晶状体上皮细胞增殖与分化的影响以及异常增殖与分化在激素性白内障形成中的作用。方法:对大鼠晶状体上皮细胞进行原代培养,应用不同浓度的地塞米松作用于2代上皮细胞24h,采用MTT方法检测地塞米松对上皮细胞增殖的影响,采用RT-PCR和Westernblotting方法,在转录及蛋白水平检测晶状体上皮细胞分化标志蛋白(β-晶状体蛋白)表达的变化,了解地塞米松对上皮细胞分化的影响。结果:地塞米松在一定范围内对晶状体上皮细胞有促进增殖的作用,其增殖率分别为10-8mol/L组131.9%,10-7mol/L组142.5%,10-6mol/L组183.4%;地塞米松在转录及蛋白水平可降低晶状体上皮细胞中β-晶状体蛋白的表达,在转录水平的表达分别为对照组2.07±0.26,10-8mol/L组1.48±0.07,10-7mol/L组1.06±0.16,10-6mol/L组0.78±0.16,在蛋白水平的表达分别为对照组1.38±0.16,10-8mol/L组1.07±0.11,10-7mol/L组0.97±0.04,10-6mol/L组0.62±0.12,说明地塞米松对晶状体上皮细胞的分化有抑制作用。结论:地塞米松对晶状体上皮细胞促进增殖和抑制分化的作用可能在激素性白内障形成机制中有一定的作用。AIM： To assess the effect of dexamethasone on proliferation and differentiation of rat lens epithelial cells in vitro and to investigate the mechanism of gluco-corticoid induced cataract. METHODS： Primary rat lens epithelial cells （rLECs） cultures were used throughout this study. The second generation rLECs were exposed to different concen-trations of dexamethasone for 24 hours. Proliferation feature of rLECs was detected with MTT assay. RT-PCR and Western blotting were applied to identify the expression of β-crystallin, specific marker of lens epithelial cells differentiation, at protein and transcription levels. RESULTS： Dexamethasone induced proliferation of rLECs in certain scope. The rate of proliferation was 131.9% （10^-8mol/L group）, 142.5% （10^-7mol/L group） and 183.4% （10^-6mol/L group） respectively, rLECs treated by dexamethasone showed a decline in β-crystallin expression at protein and transcription levels. The expression of β-crystallin mRNA was 2.07 ± 0.26 （ control group）, 1.84±0.07 （10^-8mol/L group）, 1.06±0.16 （10^-7mol/L group）, and 0. 78±0.16（10^-6mol/L group） at transcription level. The expression of β-crystallin was 1.38±0.16 （control group）, 1.07±0.11 （10^-8mol/L group）, 0.97±0.04 （10^-7mol/L group）, and 0.62±0.12 （10^-6mol/L group） at protein level. It showed that dexamethasone had inhibitive effects on differentiation of lens epithelial cells. CONCLUSION： Proliferation enhancement and differentiation inhibition of dexamethasone-treated lens epithelial cells may play an important role in the development of glucocorticoid induced cataract.

关 键 词：地塞米松 激素性白内障 晶状体上皮细胞 增殖分化 

分 类 号：R776.1[医药卫生—眼科] R714.51[医药卫生—临床医学]