小鼠睡眠生物解析系统与应用  

Somnogenic effects of L-stepholidine in mice

作　　者：邱梅红[1] 岳小芳[1] 徐昕红[2] 黄志力[1] 

机构地区：[1]复旦大学上海医学院医学神经生物学国家重点实验室,上海200032 [2]复旦大学上海医学院药理学系,上海200032

出　　处：《中国药理学通报》2008年第1期20-23,共4页Chinese Pharmacological Bulletin

基　　金：国家自然科学基金资助项目(No30570581,30625021);上海市浦江计划资助项目(No06PJ14008)

摘　　要：目的建立高度自动化小鼠睡眠生物解析系统,并考察作用于多巴胺D1/D2受体的左旋千金藤啶碱(L-stepholi-dine,SPD)对小鼠睡眠的影响。方法动物饲养在恒温、恒湿、隔音、静电屏蔽、12h/12h明暗光照环境下,利用Sleep-Sign软件,连续48h记录自由运动小鼠的脑电和肌电,分别在d121:00点腹腔注射生理盐水和d2同一时间注射SPD;并用此软件对脑电和肌电进行分析,自动判断睡眠-觉醒时相。结果该系统能高度自动化和高效率地记录与解析小鼠睡眠-觉醒行为。小鼠觉醒期腹腔注射SPD能增加睡眠量,延长非快动眼睡眠波的时程,降低觉醒量及觉醒期脑电波的强度,但不影响快动眼睡眠。结论作用于多巴胺受体的SPD能促进非快动眼睡眠,抑制觉醒。Aim To set up a highly effective and auto- matic mouse sleep-wake bioassay system, and evaluate the system through analysis of the somnogenic effects of L-stepholidine （SPD）, targeting at dopamine D1/D2 receptors in mice. Methods The animals were housed in an insulated and soundproof recording chamber maintained at a constant temperature and humidity on an automatically controlled 12 h light/12 h dark cycle. The electroencephalogram and electromyogram were recorded continuously for 48 hours and analyzed by SleepSign software. Saline was administered ip to the mice at 21：00 on the first day, and SPD was given on the next day at the same hour. The vigilance state was analyzed based on the polygraphic recordings by the same software. Results The system has been demonstrated to be highly efficient and stable in recording and reliable in analyzing sleep-wake behavior in mice. With the aid of the sleep bioassay system, we found that SPD significantly increased the total time spent in sleep during dark period, and prolonged durations of non-rapid eye movement sleep episodes, with a concomitant reduction in amount and EEG power density of wakefulness. SPD rendered no effect on rapid eye movement sleep. Conclusion Through the reliable mouse sleep bioassay system, we found that SPD promotes non-rapid eye movement sleep but not rapid eye movement sleep in mice.

关 键 词：睡眠 脑电图 肌电图 L-stepholidine 多巴胺受体拮抗剂 

分 类 号：R-332[医药卫生] R284.1