CGRP对局灶性脑缺血再灌注大鼠顶叶皮质CREB和p-CREB的上调作用  被引量:6

Upregulative effect of CGRP on expression of CREB and phospho-CREB protein during focal cerebral ischemia/reperfusion in rat parietal cortex

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作  者:张正洪[1] 肖红菊[2] 席刚明[3] 曲鹏[4] 方秀斌[4] 

机构地区:[1]郧阳医学院解剖学教研室 [2]郧阳医学院附属东风公司总医院内三科,湖北十堰442000 [3]郧阳医学院附属人民医院神经内科 [4]中国医科大学基础医学院神经生物学教研室,辽宁沈阳110001

出  处:《中国病理生理杂志》2008年第1期28-31,共4页Chinese Journal of Pathophysiology

基  金:辽宁省自然科学基金资助项目(No.619019)

摘  要:目的:探讨外源性降钙素基因相关肽(CGRP)对局灶性脑缺血再灌注大鼠顶叶皮质CREB和磷酸化CREB(p-CREB)表达的影响。方法:用线栓法阻塞大鼠右大脑中动脉制作局灶性脑缺血再灌注模型,应用免疫组织化学、Western blotting和图像分析方法检测大鼠手术侧顶叶皮质CREB和p-CREB表达。结果:缺血再灌注组大鼠顶叶皮质CREB表达少于假手术组,CGRP组大鼠顶叶皮质CREB表达多于缺血再灌注组(P<0.05)。假手术组右侧顶叶皮质p-CREB表达很少,缺血再灌注组顶叶皮质p-CREB表达多于假手术组,CGRP组p-CREB表达多于缺血再灌注组(P<0.05)。结论:CGRP上调局灶性脑缺血再灌注大鼠右侧顶叶皮质CREB和p-CREB的表达,CGRP对缺血神经元的保护作用可能是通过上调神经元内CREB和p-CREB来实现的。AIM: To investigate the effect of calcitonin gene related peptide (CGRP) on the expression of cyclic AMP response element binding protein (CREB) and phosphorylated -CREB in rat parietal cortex during focal cerebral ischemia/reperfusion (I/R). METHODS: Focal cerebral ischemia/reperfusion model was induced by occlusion of the right middle cerebral artery using the intraluminal suture method. The expressions of CREB and phospho - CREB in the parietal cortex in different groups (sham group, focal cerebral ischemia/reperfusion group and CGRP group) were detected with immunohistochemistry and Western blotting, and the positive products were analyzed by image analysis system. RESULTS: There was definite expression of CREB in right parietal cortex in sham group, while it was lesser in I/R group than that in sham group, but it became more in CGRP group than that in I/R group ( P 〈 0. 05 ). Phospho - CREB was barely detected in right parietal cortex in sham group and it became more in I/R group than that in sham group. The expression of phospho - CREB increased in CGRP group than that in I/R group of the right parietal cortex ( P 〈 0. 05 ). CONCLUSION: CGRP upregulates the expression of CREB and phospho - CREB in the ischemic neurons of the parietal cortex during focal cerebral ischemia/reperfusion and CREB probably involves in the mechanism of protective role of CGRP to ischemic neurons.

关 键 词:脑缺血 降钙素基因相关肽 c—AMP反应元件结合蛋白 大鼠 顶叶 

分 类 号:R743[医药卫生—神经病学与精神病学] R322.81[医药卫生—临床医学]

 

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