人参二醇组皂苷对LPS致休克大鼠肝脏TLR2、TLR9 mRNA表达的影响  

Effects of panaxadiol saponin on TLR2 and TLR9 mRNA expression in LPS induced shock rats

作　　者：王志[1] 李洪岩[1] 吕文伟[2] 刘姗姗[3] 穆桂芳[1] 李扬[1] 孙连坤[1] 

机构地区：[1]吉林大学基础医学院病理生理学教研室,吉林长春130021 [2]吉林大学基础医学院药理学教研室,吉林长春130021 [3]吉林大学基础医学院吉林大学2002级临床医学,吉林长春130021

出　　处：《中国病理生理杂志》2008年第1期116-118,共3页Chinese Journal of Pathophysiology

基　　金：吉林省中医药管理局中医药科学技术研究基金(No.2004-080)

摘　　要：目的:观察人参二醇组皂苷(PDS)对TLR2和TLR9 mRNA表达的影响,探讨PDS抗休克的分子生物学机制。方法:大鼠随机分为对照(control)组、LPS休克(LPS)组、人参二醇组皂苷小剂量(LPS+PDSL)组和人参二醇组皂苷中剂量(LPS+PDSM)组。大鼠舌下静脉注射LPS(4mg/kg)4h后测定血清中NOS活性、NO含量,肝组织中LPO含量、SOD活性以及TLR2、TLR4 mRNA的表达。结果:LPS+PDSL组和LPS+PDSM组NOS活性、NO含量和LPO含量明显低于LPS组,SOD活性明显高于LPS组(P<0.05);LPS+PDSL组和LPS+PDSM组TLR2mRNA表达明显低于LPS组,TLR9 mRNA表达无变化。结论:PDS通过下调肝组织中TLR2 mRNA表达,降低NOS活性、NO含量,对肝脏有保护作用。AIM： To explore the molecular mechanism of panaxadiol saponin （PDS） by observing Toll like receptor （TLR） 2 and TLR9 mRNA expression induced by lipopolysaccharide （LPS）. METHODS： Rats were divided into LPS, LPS ＋ PDSL, LPS ＋ PDSM and control group, respectively. Nitric oxide synthase （NOS） activity, nitric oxide （NO） content, LPO content, SOD activity and TLR2 and TLR9 mRNA expression were assayed 4 h after intravenous injection of LPS. RESULTS： NOS activity, NO content, LPO content of LPS ＋ PDSL group and LPS ＋ PDSM group were significantly lower than those in LPS group. TLR2 mRNA expression in the liver tissue of LPS ＋ PDSL group and LPS ＋ PDSM group was decreased compared with LPS group. CONCLUSION： PDS has a protective effect on liver tissues by triggering the down - regulation of TLR2 expression, reducing NOS activity, and NO content.

关 键 词：脂多糖类 人参 受体 Toll样 

分 类 号：R363[医药卫生—病理学]