低剂量米非司酮对人卵巢黄素化颗粒细胞凋亡的影响  

Effects of low-dose mifepristone on apoptosis in human ovarian luteinized granulosa cells

作　　者：张武文[1] 庄亚玲[1] 王雯[1] 韦浪花[1] 黄丽丽[1] 

机构地区：[1]浙江大学医学院附属妇产科医院,浙江杭州310006

出　　处：《中国病理生理杂志》2008年第1期157-161,共5页Chinese Journal of Pathophysiology

基　　金：浙江省科技厅计划资助项目(No.2003C33013)

摘　　要：目的:观察不同低剂量米非司酮对人卵巢黄素化颗粒细胞凋亡的影响,确定米非司酮作为口服避孕药的理论基础。方法:体外培养颗粒细胞。利用免疫荧光、脱氧核糖核酸末端转移酶介导的dUTP缺口末端标记(TUNEL)及流式细胞技术检测不同低剂量米非司酮处理下颗粒细胞的凋亡形态、凋亡率及caspase-3荧光强度。结果:免疫荧光检测显示处理组中颗粒细胞呈现染色质浓缩,边缘化的凋亡形态。TUNEL表明,米非司酮处理各组均与对照组差异显著,P<0.01;米非司酮处理各组之间两两比较,除1.25μmol/L与2.50μmol/L外,均有显著差异,P<0.01。流式细胞检测发现,米非司酮各处理组中caspase-3的荧光强度与对照组均有显著差异(P<0.01),各处理组之间比较与TUNEL结果相一致。结论:低剂量米非司酮能诱导颗粒细胞凋亡,1.25μmol/L浓度的米非司酮也能诱导颗粒细胞凋亡。米非司酮可能通过影响caspase-3的活性变化来调节颗粒细胞的凋亡。AIM： To investigate the effect of different low - dose mifepristone on apoptosis in granulosa cells and to test low- dose mifepristone as an orally contraceptive drug. METHODS： By using immunofluorescence, terminal deoxynucleotidyl transferase - mediated nick end labelling （TUNEL） and flow cytometry technique, the nuclear morphologic features and ratio of apoptosis and fluorescent intensity of caspase - 3 in granulosa cells cultured in vitro treated with different low - doses of mifepristone were observed, respectively. RESULTS： By the display of immunofluorescence, the granulosa cells in treatment group were classified as apoptotic ceils on the basis of their morphologic features contained a single condensed chromatin, multiple nuclear fragments. The results of TUNEL showed significant difference between control group and groups treated with different concentration of mifepristone （ P 〈 0. 01 ）. A significant difference （P 〈 0. 01 ） was also observed between the treatment groups with 1.25 μmol/L and 2. 50 μmol/L mifepristone. The fluorescent intensity of caspase - 3, observed by flow cytometry showed significant difference （ P 〈 0.01 ） between control group and treatment groups. CONCLUSION ： Granulosa cells are induced to apoptosis by low - dose mifepristone, which may be regulated by the activation of caspase- 3.

关 键 词：米非司酮 粒层细胞 细胞凋亡 避孕 

分 类 号：R363[医药卫生—病理学]